245289

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 643884..644611 Replicon chromosome
Accession NZ_CP097367
Organism Escherichia coli strain AGR6128

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag M7747_RS03190 Protein ID WP_000550189.1
Coordinates 643884..644198 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M7747_RS03195 Protein ID WP_000560269.1
Coordinates 644195..644611 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M7747_RS03170 (640041) 640041..641027 - 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -
M7747_RS03175 (641106) 641106..641798 - 693 WP_000942551.1 vancomycin high temperature exclusion protein -
M7747_RS03180 (641875) 641875..642378 - 504 WP_001295542.1 M48 family metallopeptidase -
M7747_RS03185 (642463) 642463..643599 + 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
M7747_RS03190 (643884) 643884..644198 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
M7747_RS03195 (644195) 644195..644611 + 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
M7747_RS03200 (644656) 644656..646674 - 2019 WP_135097547.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
M7747_RS03205 (646900) 646900..649251 - 2352 WP_000695431.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T245289 WP_000550189.1 NZ_CP097367:643884-644198 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT245289 WP_000560269.1 NZ_CP097367:644195-644611 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References