Detailed information of TA system TA024519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 26841..27339
• Replicon: plasmid pCROD1
• Accession: NC_013717
• Organism: Citrobacter rodentium ICC168

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ROD_RS27865
• Protein ID: WP_081442382.1
• Coordinates: 26841..26993 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ROD_RS25145
• Protein ID: WP_024133137.1
• Coordinates: 27079..27339 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ROD_RS27195	21969..22196	-	228	Protein_22	adhesin	-
ROD_RS25135	22328..26254	-	3927	WP_012908920.1	autotransporter outer membrane beta-barrel domain-containing protein	-
ROD_RS27865	26841..26993	+	153	WP_081442382.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ROD_RS25145	27079..27339	+	261	WP_024133137.1	helix-turn-helix domain-containing protein	Antitoxin
ROD_RS27205	27643..27942	+	300	WP_024133138.1	hypothetical protein	-
ROD_RS25150	27957..30536	+	2580	WP_012908922.1	fimbrial biogenesis outer membrane usher protein	-
ROD_RS25155	30578..31048	+	471	WP_012908923.1	hypothetical protein	-
ROD_RS27870	31572..32285	+	714	WP_148222154.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pic	1..54449	54449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (2-49)

Antitoxin

• Cro/C1-type helix-turn-helix domain (27-71)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5724.90 Da
• Isoelectric Point: 10.6362

>T24519 WP_081442382.1 NC_013717:26841-26993 [Citrobacter rodentium ICC168]
MGKRGGVRVIYYVRLASGRLYLLLIYPKNAKDDLSEKEKAVMKVLAQQLK

Nucleotide

• Length: 153 bp

>T24519 NC_013717:26841-26993 [Citrobacter rodentium ICC168]
ATGGGGAAGCGAGGTGGCGTCAGAGTGATTTACTACGTTCGCCTTGCCAGCGGCAGATTATACCTGCTTCTGATATACCC
TAAGAATGCTAAAGATGATCTTAGCGAGAAAGAAAAAGCCGTTATGAAGGTATTAGCACAGCAGCTGAAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9727.37 Da
• Isoelectric Point: 7.2801

>AT24519 WP_024133137.1 NC_013717:27079-27339 [Citrobacter rodentium ICC168]
MEEMVAIENGQIQPAEENIHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLIRSWEQKRRIPSGIALKMLCLIEREPALI
NTLRSI

Nucleotide

• Length: 261 bp

>AT24519 NC_013717:27079-27339 [Citrobacter rodentium ICC168]
ATGGAGGAAATGGTTGCCATTGAGAATGGCCAAATCCAGCCAGCTGAAGAAAATATTCACCGCCATACACTGCCGGATGT
GAAGGCTATTCGTAAACATGCCGGAATGAAACAGGCTGAATTTGCAGAAGTGGTGGGAGCCAGCGTTGACCTTATCCGCA
GCTGGGAACAGAAACGGCGTATCCCGTCGGGTATTGCGTTGAAAATGCTTTGTCTCATCGAACGTGAGCCAGCACTTATT
AATACCTTGCGCAGCATTTAA