Detailed information of TA system TA024510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3479283..3479880
• Replicon: chromosome
• Accession: NC_013716
• Organism: Citrobacter rodentium ICC168

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D2TN71
• Locus tag: ROD_RS16265
• Protein ID: WP_012907383.1
• Coordinates: 3479557..3479880 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ROD_RS16260
• Protein ID: WP_024132888.1
• Coordinates: 3479283..3479570 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ROD_RS16240	3474557..3475555	-	999	WP_012907379.1	class 1 fructose-bisphosphatase	-
ROD_RS16245	3475731..3477104	+	1374	WP_012907380.1	UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase	-
ROD_RS16250	3477218..3477769	-	552	WP_012907381.1	ribosome-associated protein	-
ROD_RS16255	3477865..3479217	+	1353	WP_012907382.1	metalloprotease PmbA	-
ROD_RS16260	3479283..3479570	-	288	WP_024132888.1	helix-turn-helix transcriptional regulator	Antitoxin
ROD_RS16265	3479557..3479880	-	324	WP_012907383.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ROD_RS16270	3480310..3480648	+	339	WP_012907384.1	glycine dehydrogenase	-
ROD_RS16275	3480659..3481021	+	363	WP_000435389.1	hypothetical protein	-
ROD_RS16280	3481024..3481323	+	300	WP_012907385.1	DUF4312 family protein	-
ROD_RS16285	3481346..3482122	+	777	WP_012907386.1	DUF4311 domain-containing protein	-
ROD_RS16290	3482134..3482778	+	645	WP_012907387.1	DUF4310 family protein	-
ROD_RS16295	3483002..3483982	+	981	WP_012904434.1	IS110-like element ISCro4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3483002..3483982	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12290.70 Da
• Isoelectric Point: 10.0615

>T24510 WP_012907383.1 NC_013716:c3479880-3479557 [Citrobacter rodentium ICC168]
MFTVILHVDVAKELAELPPIVQAKMIRLIDKLKANPTVLREPDSKPLGAGLFEIRTMGIGIARGLYVYQKGKRIYLLRVF
IKKTQKTPEHEMLLAFTRLEEMLNEEN

Nucleotide

• Length: 324 bp

>T24510 NC_013716:c3479880-3479557 [Citrobacter rodentium ICC168]
ATGTTCACCGTTATATTGCATGTCGACGTCGCCAAAGAATTAGCCGAGCTGCCGCCCATTGTTCAGGCTAAAATGATCCG
CCTGATCGATAAGTTAAAAGCGAACCCCACAGTGCTTCGGGAACCGGACAGTAAGCCCCTCGGCGCGGGACTGTTTGAAA
TACGCACTATGGGGATCGGGATCGCCCGGGGCCTGTATGTTTATCAGAAAGGTAAAAGGATCTACCTGCTGCGGGTCTTT
ATTAAAAAAACGCAGAAAACGCCGGAACACGAAATGCTGTTAGCCTTCACAAGGCTGGAGGAGATGCTCAATGAAGAAAA
CTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10642.36 Da
• Isoelectric Point: 10.3485

>AT24510 WP_024132888.1 NC_013716:c3479570-3479283 [Citrobacter rodentium ICC168]
MKKTKSVAWDEVRTDLLSSPEVQAAMEAEERKARLQAMLAEWRNHAGLTRAQVAERMGVTPPTVSRMEANIVRASLETIA
RYARACGIKHPQILL

Nucleotide

• Length: 288 bp

>AT24510 NC_013716:c3479570-3479283 [Citrobacter rodentium ICC168]
ATGAAGAAAACTAAAAGTGTCGCCTGGGACGAGGTCAGAACAGACCTTCTGTCCAGTCCTGAGGTACAGGCTGCAATGGA
AGCAGAAGAACGTAAAGCGCGTCTGCAGGCTATGCTCGCAGAATGGCGCAATCATGCTGGCTTAACCCGCGCACAGGTTG
CAGAGAGAATGGGCGTCACGCCACCGACGGTATCCAGAATGGAGGCCAATATTGTCAGGGCCAGCCTGGAAACCATCGCC
CGCTATGCCAGAGCCTGTGGAATCAAACATCCACAAATCCTTTTATAA

Structures

Toxin

• AlphaFold DB: D2TN71