Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3148605..3149202 Replicon chromosome
Accession NZ_CP097237
Organism Klebsiella pneumoniae strain

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag M4268_RS15050 Protein ID WP_004142563.1
Coordinates 3148605..3148922 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag M4268_RS15055 Protein ID WP_004142561.1
Coordinates 3148915..3149202 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4268_RS15035 (3144308) 3144308..3145735 - 1428 WP_043906839.1 MFS transporter -
M4268_RS15040 (3145844) 3145844..3146710 + 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
M4268_RS15045 (3147377) 3147377..3148420 + 1044 WP_004178895.1 DUF2157 domain-containing protein -
M4268_RS15050 (3148605) 3148605..3148922 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M4268_RS15055 (3148915) 3148915..3149202 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
M4268_RS15060 (3149470) 3149470..3150120 - 651 Protein_2931 oxygen-insensitive NAD(P)H nitroreductase -
M4268_RS15065 (3150240) 3150240..3150608 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
M4268_RS15070 (3150608) 3150608..3151189 - 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
M4268_RS15075 (3151369) 3151369..3152484 + 1116 WP_020316605.1 MBL fold metallo-hydrolase -
M4268_RS15080 (3152515) 3152515..3152856 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
M4268_RS15085 (3152874) 3152874..3153122 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T245005 WP_004142563.1 NZ_CP097237:3148605-3148922 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT245005 WP_004142561.1 NZ_CP097237:3148915-3149202 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References