Detailed information of TA system TA024498

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1654368..1654930
• Replicon: chromosome
• Accession: NC_013716
• Organism: Citrobacter rodentium ICC168

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D2TJA7
• Locus tag: ROD_RS07755
• Protein ID: WP_012905816.1
• Coordinates: 1654368..1654646 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ROD_RS07760
• Protein ID: WP_012905817.1
• Coordinates: 1654646..1654930 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ROD_RS28800	1649512..1650254	+	743	Protein_1530	GntP family permease	-
ROD_RS07730	1650266..1651036	+	771	WP_012905811.1	3-hydroxybutyrate dehydrogenase	-
ROD_RS07735	1651106..1651537	-	432	WP_012905812.1	peroxiredoxin OsmC	-
ROD_RS07740	1651876..1652091	+	216	WP_012905813.1	biofilm-dependent modulation protein	-
ROD_RS07745	1652183..1652326	+	144	WP_012905814.1	stationary-phase-induced ribosome-associated protein	-
ROD_RS07750	1652486..1654183	+	1698	WP_012905815.1	malate dehydrogenase	-
ROD_RS07755	1654368..1654646	+	279	WP_012905816.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ROD_RS07760	1654646..1654930	+	285	WP_012905817.1	HigA family addiction module antidote protein	Antitoxin
ROD_RS07765	1654988..1655590	-	603	WP_012905818.1	inorganic diphosphatase	-
ROD_RS07770	1655716..1656372	-	657	WP_012905819.1	formate dehydrogenase-N subunit gamma	-
ROD_RS07775	1656365..1657249	-	885	WP_012905820.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10760.28 Da
• Isoelectric Point: 8.1172

>T24498 WP_012905816.1 NC_013716:1654368-1654646 [Citrobacter rodentium ICC168]
MIMNFRHKGLRDLFLHGQTSGVMTQHVRRLRHRLAVIDAACRISDIDMPGYRLHQLSGERDGVWAISVSGNWRITFEFIN
GDAYILNYEDYH

Nucleotide

• Length: 279 bp

>T24498 NC_013716:1654368-1654646 [Citrobacter rodentium ICC168]
ATGATTATGAATTTCCGACACAAAGGATTACGTGACCTGTTTCTTCATGGGCAAACGTCAGGCGTTATGACTCAGCACGT
TCGCCGCTTAAGGCACCGTCTGGCGGTCATCGATGCCGCCTGCCGGATTAGCGATATTGATATGCCTGGCTACCGGCTTC
ATCAGTTGTCGGGCGAGCGGGACGGTGTCTGGGCGATATCCGTTTCAGGTAACTGGCGCATCACTTTTGAATTCATCAAT
GGCGACGCGTATATATTGAATTACGAGGATTATCACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10341.12 Da
• Isoelectric Point: 10.0902

>AT24498 WP_012905817.1 NC_013716:1654646-1654930 [Citrobacter rodentium ICC168]
MKMANHPRPGEIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNAWSLAEAK
KSVDISRLRKLAVQ

Nucleotide

• Length: 285 bp

>AT24498 NC_013716:1654646-1654930 [Citrobacter rodentium ICC168]
ATGAAAATGGCAAACCATCCCCGTCCAGGGGAGATCATTCAGGAAGCGCTGGATGAACTGAACGTTAGCTTGCGTGAATT
TGCCAGAGCGATGGAAATCGCCCCCTCAACCGCCAGCCGTCTGTTAACCGGCAAGGCCGCGCTCACGCCGGAAATGGCCA
TCAAATTGTCGGTGGTGATCGGCAGTTCGCCGGAAATGTGGTTGAATCTGCAAAATGCCTGGAGCCTGGCGGAAGCAAAG
AAAAGCGTGGATATCTCACGCCTGCGTAAGCTGGCAGTACAGTAA

Structures

Toxin

• AlphaFold DB: D2TJA7