Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2201505..2202102 Replicon chromosome
Accession NZ_CP097228
Organism Klebsiella pneumoniae strain 5

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag M4270_RS10760 Protein ID WP_004142563.1
Coordinates 2201785..2202102 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag M4270_RS10755 Protein ID WP_004142561.1
Coordinates 2201505..2201792 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4270_RS10725 (2197585) 2197585..2197833 + 249 WP_002893037.1 DUF1158 domain-containing protein -
M4270_RS10730 (2197851) 2197851..2198192 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
M4270_RS10735 (2198223) 2198223..2199338 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
M4270_RS10740 (2199518) 2199518..2200099 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
M4270_RS10745 (2200099) 2200099..2200467 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
M4270_RS10750 (2200587) 2200587..2201237 + 651 Protein_2113 oxygen-insensitive NAD(P)H nitroreductase -
M4270_RS10755 (2201505) 2201505..2201792 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
M4270_RS10760 (2201785) 2201785..2202102 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M4270_RS10765 (2202287) 2202287..2203330 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
M4270_RS10770 (2203997) 2203997..2204863 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
M4270_RS10775 (2204972) 2204972..2206399 + 1428 WP_043906839.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T244970 WP_004142563.1 NZ_CP097228:c2202102-2201785 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT244970 WP_004142561.1 NZ_CP097228:c2201792-2201505 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References