Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2207241..2207838 Replicon chromosome
Accession NZ_CP097223
Organism Klebsiella pneumoniae strain 11

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag M4271_RS10785 Protein ID WP_004142563.1
Coordinates 2207521..2207838 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag M4271_RS10780 Protein ID WP_004142561.1
Coordinates 2207241..2207528 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4271_RS10750 (2203321) 2203321..2203569 + 249 WP_002893037.1 DUF1158 domain-containing protein -
M4271_RS10755 (2203587) 2203587..2203928 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
M4271_RS10760 (2203959) 2203959..2205074 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
M4271_RS10765 (2205254) 2205254..2205835 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
M4271_RS10770 (2205835) 2205835..2206203 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
M4271_RS10775 (2206323) 2206323..2206973 + 651 Protein_2118 oxygen-insensitive NAD(P)H nitroreductase -
M4271_RS10780 (2207241) 2207241..2207528 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
M4271_RS10785 (2207521) 2207521..2207838 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M4271_RS10790 (2208023) 2208023..2209066 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
M4271_RS10795 (2209733) 2209733..2210599 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
M4271_RS10800 (2210708) 2210708..2212135 + 1428 WP_043906839.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T244953 WP_004142563.1 NZ_CP097223:c2207838-2207521 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT244953 WP_004142561.1 NZ_CP097223:c2207528-2207241 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References