244920

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3782732..3783426 Replicon chromosome
Accession NZ_CP097213
Organism Escherichia coli strain BE311

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag M3O69_RS18360 Protein ID WP_001263489.1
Coordinates 3782732..3783130 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag M3O69_RS18365 Protein ID WP_000554758.1
Coordinates 3783133..3783426 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3778321) 3778321..3778401 - 81 NuclAT_11 - -
- (3778321) 3778321..3778401 - 81 NuclAT_11 - -
- (3778321) 3778321..3778401 - 81 NuclAT_11 - -
- (3778321) 3778321..3778401 - 81 NuclAT_11 - -
M3O69_RS18335 (3778997) 3778997..3779455 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
M3O69_RS18340 (3779716) 3779716..3781173 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
M3O69_RS18345 (3781230) 3781230..3781750 - 521 Protein_3588 peptide chain release factor H -
M3O69_RS18350 (3781746) 3781746..3781952 - 207 Protein_3589 RtcB family protein -
M3O69_RS18355 (3782270) 3782270..3782722 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
M3O69_RS18360 (3782732) 3782732..3783130 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M3O69_RS18365 (3783133) 3783133..3783426 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M3O69_RS18370 (3783478) 3783478..3784533 - 1056 WP_001226164.1 DNA polymerase IV -
M3O69_RS18375 (3784604) 3784604..3785389 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
M3O69_RS18380 (3785361) 3785361..3787073 + 1713 Protein_3595 flagellar biosynthesis protein FlhA -
M3O69_RS18385 (3787297) 3787297..3787794 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T244920 WP_001263489.1 NZ_CP097213:c3783130-3782732 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT244920 WP_000554758.1 NZ_CP097213:c3783426-3783133 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References