Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3716261..3716802 Replicon chromosome
Accession NZ_CP097181
Organism Escherichia coli strain SH21PTE31

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag M4R35_RS18280 Protein ID WP_000615983.1
Coordinates 3716524..3716802 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag M4R35_RS18275 Protein ID WP_000729703.1
Coordinates 3716261..3716521 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4R35_RS18255 (3711937) 3711937..3712722 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
M4R35_RS18260 (3712694) 3712694..3714406 + 1713 Protein_3593 flagellar biosynthesis protein FlhA -
M4R35_RS18265 (3714630) 3714630..3715127 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
M4R35_RS18270 (3715303) 3715303..3716051 - 749 Protein_3595 C40 family peptidase -
M4R35_RS18275 (3716261) 3716261..3716521 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
M4R35_RS18280 (3716524) 3716524..3716802 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
M4R35_RS18285 (3716958) 3716958..3717698 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
M4R35_RS18290 (3717669) 3717669..3718436 - 768 WP_000333380.1 class II glutamine amidotransferase -
M4R35_RS18295 (3718642) 3718642..3719220 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T244876 WP_000615983.1 NZ_CP097181:3716524-3716802 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT244876 WP_000729703.1 NZ_CP097181:3716261-3716521 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References