Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3710065..3710759 Replicon chromosome
Accession NZ_CP097181
Organism Escherichia coli strain SH21PTE31

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag M4R35_RS18240 Protein ID WP_001263493.1
Coordinates 3710065..3710463 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag M4R35_RS18245 Protein ID WP_000554757.1
Coordinates 3710466..3710759 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4R35_RS18210 (3705624) 3705624..3706082 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
M4R35_RS18215 (3706343) 3706343..3707800 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
M4R35_RS18220 (3707857) 3707857..3708378 - 522 Protein_3585 peptide chain release factor H -
M4R35_RS18225 (3708377) 3708377..3708580 - 204 Protein_3586 RtcB family protein -
M4R35_RS18230 (3708826) 3708826..3709278 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
M4R35_RS18235 (3709352) 3709352..3710049 + 698 WP_223367350.1 IS1 family transposase -
M4R35_RS18240 (3710065) 3710065..3710463 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M4R35_RS18245 (3710466) 3710466..3710759 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M4R35_RS18250 (3710811) 3710811..3711866 - 1056 WP_001226164.1 DNA polymerase IV -
M4R35_RS18255 (3711937) 3711937..3712722 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
M4R35_RS18260 (3712694) 3712694..3714406 + 1713 Protein_3593 flagellar biosynthesis protein FlhA -
M4R35_RS18265 (3714630) 3714630..3715127 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3709756..3710049 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T244875 WP_001263493.1 NZ_CP097181:c3710463-3710065 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT244875 WP_000554757.1 NZ_CP097181:c3710759-3710466 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References