Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4140165..4140706 Replicon chromosome
Accession NZ_CP096976
Organism Escherichia coli strain 17-07187

Toxin (Protein)


Gene name yafQ Uniprot ID B7LHF6
Locus tag M2894_RS20325 Protein ID WP_000615974.1
Coordinates 4140428..4140706 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag M2894_RS20320 Protein ID WP_000729704.1
Coordinates 4140165..4140425 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2894_RS20295 (4135948) 4135948..4136733 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
M2894_RS20300 (4136705) 4136705..4138417 + 1713 Protein_3977 flagellar biosynthesis protein FlhA -
M2894_RS20305 (4138522) 4138522..4138800 + 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
M2894_RS20310 (4138793) 4138793..4139149 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
M2894_RS20315 (4139206) 4139206..4139979 - 774 WP_000543895.1 C40 family peptidase -
M2894_RS20320 (4140165) 4140165..4140425 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
M2894_RS20325 (4140428) 4140428..4140706 + 279 WP_000615974.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
M2894_RS20330 (4140862) 4140862..4141602 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
M2894_RS20335 (4141573) 4141573..4142340 - 768 WP_000333380.1 class II glutamine amidotransferase -
M2894_RS20340 (4142546) 4142546..4143124 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10855.68 Da        Isoelectric Point: 10.2267

>T244330 WP_000615974.1 NZ_CP096976:4140428-4140706 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMRLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT244330 WP_000729704.1 NZ_CP096976:4140165-4140425 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y514

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References