Detailed information of TA system TA024422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2482550..2483067
• Replicon: chromosome
• Accession: NC_013450
• Organism: Staphylococcus aureus subsp. aureus ED98

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: SAAV_RS12710
• Protein ID: WP_000113265.1
• Coordinates: 2482550..2482816 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: SAAV_RS12715
• Protein ID: WP_000584499.1
• Coordinates: 2482816..2483067 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAAV_RS12685	2477978..2478514	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
SAAV_RS12690	2478748..2479572	-	825	WP_000572038.1	formate/nitrite transporter family protein	-
SAAV_RS12695	2479789..2479971	-	183	WP_000230292.1	hypothetical protein	-
SAAV_RS12700	2480055..2480522	-	468	WP_000153347.1	SRPBCC domain-containing protein	-
SAAV_RS12705	2480708..2482255	-	1548	WP_000727774.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
SAAV_RS15085	2482444..2482515	-	72	Protein_2404	hypothetical protein	-
SAAV_RS12710	2482550..2482816	-	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
SAAV_RS12715	2482816..2483067	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SAAV_RS12720	2483232..2483333	-	102	Protein_2407	hypothetical protein	-
SAAV_RS12725	2483340..2483939	-	600	WP_000162810.1	DsbA family protein	-
SAAV_RS12730	2483958..2484320	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
SAAV_RS12735	2484575..2485825	+	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
SAAV_RS12740	2485922..2486653	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
SAAV_RS12745	2486650..2487369	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T24422 WP_000113265.1 NC_013450:c2482816-2482550 [Staphylococcus aureus subsp. aureus ED98]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T24422 NC_013450:c2482816-2482550 [Staphylococcus aureus subsp. aureus ED98]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT24422 WP_000584499.1 NC_013450:c2483067-2482816 [Staphylococcus aureus subsp. aureus ED98]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT24422 NC_013450:c2483067-2482816 [Staphylococcus aureus subsp. aureus ED98]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60