Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5450231..5450826 Replicon chromosome
Accession NZ_CP096912
Organism Pseudomonas aeruginosa strain NY7770

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag M2J78_RS25360 Protein ID WP_003113526.1
Coordinates 5450548..5450826 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag M2J78_RS25355 Protein ID WP_003111575.1
Coordinates 5450231..5450536 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2J78_RS25325 (M2J78_25295) 5445588..5446343 + 756 WP_074198791.1 IS21-like element ISPpu7 family helper ATPase IstB -
M2J78_RS25330 (M2J78_25300) 5446693..5447346 - 654 WP_073651898.1 hypothetical protein -
M2J78_RS25335 (M2J78_25305) 5447490..5447729 + 240 WP_011806687.1 helix-turn-helix transcriptional regulator -
M2J78_RS25340 (M2J78_25310) 5448458..5449330 - 873 WP_212591743.1 plasmid replication initiator TrfA -
M2J78_RS25345 (M2J78_25315) 5449366..5449578 - 213 WP_058133021.1 AlpA family phage regulatory protein -
M2J78_RS25355 (M2J78_25325) 5450231..5450536 - 306 WP_003111575.1 HigA family addiction module antitoxin Antitoxin
M2J78_RS25360 (M2J78_25330) 5450548..5450826 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M2J78_RS25365 (M2J78_25335) 5450879..5451007 - 129 Protein_5011 integrase -
M2J78_RS25370 (M2J78_25340) 5451155..5453383 + 2229 WP_021264080.1 TonB-dependent receptor -
M2J78_RS25375 (M2J78_25345) 5453453..5454100 - 648 WP_003095021.1 carbonate dehydratase -
M2J78_RS25380 (M2J78_25350) 5454162..5455400 - 1239 WP_003111578.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T244156 WP_003113526.1 NZ_CP096912:c5450826-5450548 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT244156 WP_003111575.1 NZ_CP096912:c5450536-5450231 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References