Detailed information of TA system TA024408

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF1/-
• Location: 892733..893227
• Replicon: chromosome
• Accession: NC_013450
• Organism: Staphylococcus aureus subsp. aureus ED98

Toxin (Protein)

• Gene name: holin
• Uniprot ID: A0A4P7P563
• Locus tag: SAAV_RS04525
• Protein ID: WP_000351119.1
• Coordinates: 892952..893227 (+)
• Length: 92 a.a.

Antitoxin (RNA)

Gene name	SprF1
Locus tag	-
Coordinates	892733..892872 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAAV_RS04500	891507..891659	+	153	WP_001000058.1	hypothetical protein	-
SAAV_RS04505	891705..891992	+	288	WP_001262623.1	hypothetical protein	-
SAAV_RS04510	892048..892422	+	375	WP_000340977.1	hypothetical protein	-
SAAV_RS14780	892565..892741	+	177	WP_072353918.1	putative holin-like toxin	-
-	892733..892872	-	140	NuclAT_0	-	Antitoxin
-	892733..892872	-	140	NuclAT_0	-	Antitoxin
-	892733..892872	-	140	NuclAT_0	-	Antitoxin
-	892733..892872	-	140	NuclAT_0	-	Antitoxin
SAAV_RS04520	892794..892901	-	108	WP_031762631.1	hypothetical protein	-
SAAV_RS04525	892952..893227	+	276	WP_000351119.1	phage holin	Toxin
SAAV_RS04530	893214..894626	+	1413	WP_001141512.1	N-acetylmuramoyl-L-alanine amidase	-
SAAV_RS04535	894686..895243	-	558	WP_001035620.1	DUF4888 domain-containing protein	-
SAAV_RS04540	895618..895770	+	153	WP_001788502.1	hypothetical protein	-
SAAV_RS04545	895841..895951	+	111	WP_000139423.1	hypothetical protein	-
SAAV_RS04550	895953..896138	+	186	WP_001286805.1	hypothetical protein	-
SAAV_RS04555	896823..896962	+	140	Protein_868	hypothetical protein	-
SAAV_RS04560	896968..897282	-	315	WP_000274029.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	851600..896138	44538

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (1-71)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 9975.53 Da
• Isoelectric Point: 9.8179

>T24408 WP_000351119.1 NC_013450:892952-893227 [Staphylococcus aureus subsp. aureus ED98]
MDIGTIVRTILLIVAWINQFLAIKHISPIPVDEVFISTVVTGIVSIWTWWKNNNFTHASKKGQQKIYEVKAGIQSTGGAP
KVNGDDNNAVG

Nucleotide

• Length: 276 bp

>T24408 NC_013450:892952-893227 [Staphylococcus aureus subsp. aureus ED98]
ATGGATATCGGTACAATCGTAAGAACAATTTTATTAATAGTCGCATGGATCAATCAGTTTTTAGCAATCAAACATATTTC
TCCAATCCCAGTTGACGAAGTGTTTATAAGCACAGTCGTTACTGGGATTGTTTCAATTTGGACTTGGTGGAAGAATAACA
ACTTTACTCACGCATCTAAGAAGGGACAACAAAAAATCTATGAAGTAAAAGCTGGCATTCAGTCAACTGGTGGCGCACCT
AAAGTGAACGGAGATGATAATAATGCCGTCGGTTAG

Antitoxin

Nucleotide

• Length: 140 bp

>AT24408 NC_013450:c892872-892733 [Staphylococcus aureus subsp. aureus ED98]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTT

Structures

Toxin

• AlphaFold DB: A0A4P7P563

Antitoxin