Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3500699..3501369 Replicon chromosome
Accession NZ_CP096848
Organism Mycobacterium tuberculosis variant bovis strain Mb0486

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag M1778_RS16520 Protein ID WP_003899954.1
Coordinates 3500699..3501043 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag M1778_RS16525 Protein ID WP_003899955.1
Coordinates 3501040..3501369 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1778_RS16485 (M1778_16480) 3495772..3496632 + 861 WP_003416628.1 alpha/beta hydrolase -
M1778_RS16490 (M1778_16485) 3496607..3497122 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
M1778_RS16495 (M1778_16490) 3497138..3497349 + 212 Protein_3256 (R)-hydratase -
M1778_RS16500 (M1778_16495) 3497362..3497655 + 294 WP_003416635.1 hypothetical protein -
M1778_RS16505 (M1778_16500) 3497943..3499232 + 1290 WP_003416640.1 ATP-binding protein -
M1778_RS16510 (M1778_16505) 3499579..3500013 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
M1778_RS16515 (M1778_16510) 3500016..3500468 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
M1778_RS16520 (M1778_16515) 3500699..3501043 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M1778_RS16525 (M1778_16520) 3501040..3501369 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
M1778_RS16530 (M1778_16525) 3501907..3502254 + 348 WP_003899956.1 DUF2384 domain-containing protein -
M1778_RS16535 (M1778_16530) 3502251..3502871 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
M1778_RS16540 (M1778_16535) 3503031..3504296 - 1266 WP_003909837.1 hypothetical protein -
M1778_RS16545 (M1778_16540) 3504464..3504673 + 210 WP_003416778.1 hypothetical protein -
M1778_RS16550 (M1778_16545) 3504920..3505954 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T244075 WP_003899954.1 NZ_CP096848:3500699-3501043 [Mycobacterium tuberculosis variant bovis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT244075 WP_003899955.1 NZ_CP096848:3501040-3501369 [Mycobacterium tuberculosis variant bovis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References