Detailed information of TA system TA024390

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 438451..439169
• Replicon: chromosome
• Accession: NC_013421
• Organism: Pectobacterium wasabiae WPP163

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PECWA_RS02020
• Protein ID: WP_012822194.1
• Coordinates: 438451..438756 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PECWA_RS02025
• Protein ID: WP_012822195.1
• Coordinates: 438753..439169 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PECWA_RS01990	433868..434602	+	735	WP_012822189.1	type I-E CRISPR-associated protein Cas5/CasD	-
PECWA_RS01995	434602..435249	+	648	WP_012822190.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
PECWA_RS02000	435277..436194	+	918	WP_012822191.1	type I-E CRISPR-associated endonuclease Cas1	-
PECWA_RS02005	436195..436488	+	294	WP_005973730.1	type I-E CRISPR-associated endoribonuclease Cas2	-
PECWA_RS02010	437595..437966	-	372	WP_012822192.1	helix-turn-helix domain-containing protein	-
PECWA_RS02015	438040..438300	-	261	WP_012822193.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PECWA_RS02020	438451..438756	+	306	WP_012822194.1	type II toxin-antitoxin system HigB family toxin	Toxin
PECWA_RS02025	438753..439169	+	417	WP_012822195.1	helix-turn-helix domain-containing protein	Antitoxin
PECWA_RS02030	440027..440485	+	459	WP_012822197.1	hypothetical protein	-
PECWA_RS02035	440566..441918	-	1353	WP_012822198.1	aspartate aminotransferase family protein	-
PECWA_RS02040	441949..443220	-	1272	WP_043988891.1	MFS transporter	-
PECWA_RS02045	443375..443821	+	447	WP_012822200.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (18-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11971.75 Da
• Isoelectric Point: 9.8538

>T24390 WP_012822194.1 NC_013421:438451-438756 [Pectobacterium parmentieri WPP163]
VFSRAPFDTAIAQFPNQAAALADLYRTIKREIYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKIFI
KHITFHAEYGSLTEYYRGNKE

Nucleotide

• Length: 306 bp

>T24390 NC_013421:438451-438756 [Pectobacterium parmentieri WPP163]
GTGTTCTCCCGTGCACCTTTTGACACTGCAATCGCCCAATTCCCTAATCAGGCGGCAGCACTGGCTGACCTATACCGGAC
GATAAAGCGTGAAATATATGCAACGCCGGACGATATGAAAAAACGTTTCCCCAGCTTAGACAGGATGAAGTACAGGGAAA
AGTGGTGGGTTATTGATATTGGTGGTGGGCATCTTCGGGTGATGTTTTTCGCTGATTTTGAGCGAGGGAAAATTTTTATC
AAACACATCACATTCCATGCAGAGTACGGCAGTCTGACAGAATATTACCGGGGGAATAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15274.52 Da
• Isoelectric Point: 4.4634

>AT24390 WP_012822195.1 NC_013421:438753-439169 [Pectobacterium parmentieri WPP163]
MMYADAIKAANNLTSIVPLLGGSTSRKDYEDALKLTEYLLESEPDSPLIDMLTAKIDKYEEDAPEFVAFNERIKALPAGI
ALLRILMDQHGLTQSDFEAEIGKKSLVSRILNGERTLTVDHMSALAKRFDIPVSAFVD

Nucleotide

• Length: 417 bp

>AT24390 NC_013421:438753-439169 [Pectobacterium parmentieri WPP163]
ATGATGTACGCAGACGCAATCAAGGCCGCTAACAATCTGACGAGTATCGTACCGCTCCTCGGAGGTAGCACCTCTCGTAA
AGATTATGAGGATGCACTGAAACTCACTGAATATTTACTCGAGAGCGAGCCTGATAGCCCGCTAATCGATATGCTGACGG
CTAAAATTGACAAATATGAAGAGGATGCGCCAGAGTTTGTAGCTTTCAACGAGCGTATTAAAGCGCTCCCTGCTGGTATC
GCTTTGCTGCGCATACTTATGGATCAGCATGGGCTAACGCAAAGTGACTTTGAAGCTGAGATCGGTAAAAAATCTCTGGT
ATCCCGCATTCTAAATGGTGAGCGTACACTAACGGTTGATCACATGAGCGCACTTGCTAAACGATTTGATATTCCCGTGA
GTGCTTTTGTTGATTAG