Detailed information of TA system TA243707

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2250286..2251205
• Replicon: chromosome
• Accession: NZ_CP096842
• Organism: Mycobacterium tuberculosis variant bovis strain Mb3114

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: M1772_RS10565
• Protein ID: WP_273590098.1
• Coordinates: 2250600..2251205 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: M1772_RS10560
• Protein ID: WP_003410124.1
• Coordinates: 2250286..2250591 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1772_RS10530 (M1772_10525)	2245297..2246553	-	1257	WP_003410095.1	HNH endonuclease signature motif containing protein	-
M1772_RS10535 (M1772_10530)	2246907..2247482	+	576	WP_003410100.1	hypothetical protein	-
M1772_RS10540 (M1772_10535)	2247479..2248519	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
M1772_RS10545 (M1772_10540)	2248761..2249480	+	720	WP_010950643.1	DUF433 domain-containing protein	-
M1772_RS10550 (M1772_10545)	2249470..2249886	+	417	WP_003410114.1	hypothetical protein	-
M1772_RS10555 (M1772_10550)	2249902..2250201	-	300	WP_003410120.1	hypothetical protein	-
M1772_RS10560 (M1772_10555)	2250286..2250591	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
M1772_RS10565 (M1772_10560)	2250600..2251205	-	606	WP_273590098.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M1772_RS10570 (M1772_10565)	2251230..2251589	-	360	WP_003410131.1	hypothetical protein	-
M1772_RS10575 (M1772_10570)	2251749..2252339	-	591	WP_057145698.1	hypothetical protein	-
M1772_RS10580 (M1772_10575)	2252378..2252614	-	237	WP_003410133.1	hypothetical protein	-
M1772_RS10585 (M1772_10580)	2252821..2253717	-	897	WP_011799238.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22668.86 Da
• Isoelectric Point: 7.3078

>T243707 WP_273590098.1 NZ_CP096842:c2251205-2250600 [Mycobacterium tuberculosis variant bovis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSSPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T243707 NZ_OX460910:c1032049-1031789 [Photobacterium phosphoreum]
ATGAGTCGAATGTTAGCTTGGACTGACGATGCTTGGGATGATTACTTGTATTGGCAAGGTCAGGATAAGAAAACGCTGAA
GCGCATCAATAAATTAATTACTGATACGAAGCGTTCTCCCTTCGAAGGCATTGGTAAACCAGAACCATTGAAAGAAAATT
TGGCAGGTTTTTGGTCACGTAGAATTGATGATACCAACCGATTAGTTTATGTAGTTAATGATGCACATTTGACAATAATT
TCCTGTCGCTATCATTATTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT243707 WP_003410124.1 NZ_CP096842:c2250591-2250286 [Mycobacterium tuberculosis variant bovis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT243707 NZ_OX460910:c1032297-1032046 [Photobacterium phosphoreum]
ATGCGTATAGTTTCTTTTACTGAAGCTCGAAATGGCCTTAAATCTGTTCTTGATGGCGTAGTTAATGATGCAGATTGCAC
AATTATCACTCGTCGAGATTCTGAAGATGCAGTTGTAATGTCTATGGATTATTATAATAGCTTAATGGAAACGATTTATT
TATCTCGTTCTCCTGCTAATGCTGCTCATCTAAACAAATCAATTGCTCAATACAATGCCGGTCAAACTACGGAAAGAGAT
TTACTTGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BV30