Detailed information of TA system TA024370

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40114..40384
• Replicon: plasmid pO111_3
• Accession: NC_013366
• Organism: Escherichia coli O111:H- str. 11128

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECO111_RS29880
• Protein ID: WP_001312861.1
• Coordinates: 40226..40384 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	40114..40177 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO111_RS34005	35133..35435	+	303	WP_077625669.1	hypothetical protein	-
ECO111_RS29850	35908..36435	+	528	WP_000290792.1	single-stranded DNA-binding protein	-
ECO111_RS29855	36491..36724	+	234	WP_000006004.1	DUF905 domain-containing protein	-
ECO111_RS29860	36783..38741	+	1959	WP_001145472.1	ParB/RepB/Spo0J family partition protein	-
ECO111_RS29865	38796..39230	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
ECO111_RS29870	39227..39946	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
ECO111_RS34110	39958..40146	-	189	WP_001299721.1	hypothetical protein	-
-	39958..40182	+	225	NuclAT_0	-	-
-	39958..40182	+	225	NuclAT_0	-	-
-	39958..40182	+	225	NuclAT_0	-	-
-	39958..40182	+	225	NuclAT_0	-	-
-	40114..40177	-	64	-	-	Antitoxin
ECO111_RS29880	40226..40384	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
ECO111_RS34195	40738..40962	-	225	WP_001427866.1	hypothetical protein	-
ECO111_RS29895	41306..41593	+	288	WP_000107535.1	hypothetical protein	-
ECO111_RS29900	41714..42535	+	822	WP_001234469.1	DUF945 domain-containing protein	-
ECO111_RS29905	42832..43341	-	510	Protein_51	transglycosylase SLT domain-containing protein	-
ECO111_RS29910	43755..44138	+	384	WP_001151524.1	relaxosome protein TraM	-
ECO111_RS29915	44325..45014	+	690	WP_000283394.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD / espP	1..77690	77690

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T24370 WP_001312861.1 NC_013366:40226-40384 [Escherichia coli O111:H- str. 11128]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T24370 NC_013366:40226-40384 [Escherichia coli O111:H- str. 11128]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT24370 NC_013366:c40177-40114 [Escherichia coli O111:H- str. 11128]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin