243665

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3504876..3505546 Replicon chromosome
Accession NZ_CP096841
Organism Mycobacterium tuberculosis variant bovis strain Mb0820

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag M1774_RS16535 Protein ID WP_003899954.1
Coordinates 3504876..3505220 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag M1774_RS16540 Protein ID WP_003899955.1
Coordinates 3505217..3505546 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1774_RS16500 (M1774_16500) 3499949..3500809 + 861 WP_003416628.1 alpha/beta hydrolase -
M1774_RS16505 (M1774_16505) 3500784..3501299 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
M1774_RS16510 (M1774_16510) 3501315..3501526 + 212 Protein_3259 (R)-hydratase -
M1774_RS16515 (M1774_16515) 3501539..3501832 + 294 WP_003416635.1 hypothetical protein -
M1774_RS16520 (M1774_16520) 3502120..3503409 + 1290 WP_003416640.1 ATP-binding protein -
M1774_RS16525 (M1774_16525) 3503756..3504190 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
M1774_RS16530 (M1774_16530) 3504193..3504645 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
M1774_RS16535 (M1774_16535) 3504876..3505220 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M1774_RS16540 (M1774_16540) 3505217..3505546 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
M1774_RS16545 (M1774_16545) 3506084..3506431 + 348 WP_003899956.1 DUF2384 domain-containing protein -
M1774_RS16550 (M1774_16550) 3506428..3507048 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
M1774_RS16555 (M1774_16555) 3507208..3508473 - 1266 WP_003909837.1 hypothetical protein -
M1774_RS16560 (M1774_16560) 3508641..3508850 + 210 WP_003416778.1 hypothetical protein -
M1774_RS16565 (M1774_16565) 3509097..3510131 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T243665 WP_003899954.1 NZ_CP096841:3504876-3505220 [Mycobacterium tuberculosis variant bovis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T243665 NZ_OX419580:2322127-2322243 [Bacillus subtilis]
ATGACTGTTTACGAATCATTAATGATAATGATCAATTTTGGCGGATTGATATTAAATACCGTCTTGTTGATCTTCAATAT
AATGATGATTGTAACGTCAAGCCAAAAGAAAAAATAG

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT243665 WP_003899955.1 NZ_CP096841:3505217-3505546 [Mycobacterium tuberculosis variant bovis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT243665 NZ_OX419580:c2322417-2322238 [Bacillus subtilis]
AATGATACAATTATATAATTATTTTTTGCATTTTGTTTAATTAAATGCATAAAATAAAAAGACCAGGGTGTTGGTAGCAC
CCTGGCTCTGTACAAAAGCTGCCCATCAAAGGGCTTGCTCGTTGTCAAATCTGCGTAGGCCTAACCCTTCAGGTGTCCAA
ACTCAAGGGAAGGTCTATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
AlphaFold DB G0THF6

References