Detailed information of TA system TA024366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4759341..4759943
• Replicon: chromosome
• Accession: NC_013364
• Organism: Escherichia coli O111:H- str. 11128

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: ECO111_RS24700
• Protein ID: WP_000897305.1
• Coordinates: 4759341..4759652 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECO111_RS24705
• Protein ID: WP_000356397.1
• Coordinates: 4759653..4759943 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO111_RS24670	4754371..4755156	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
ECO111_RS24675	4755255..4755854	+	600	WP_001315111.1	glucose-1-phosphatase	-
ECO111_RS24680	4755848..4756720	+	873	WP_000920762.1	virulence factor BrkB family protein	-
ECO111_RS24685	4756717..4757154	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ECO111_RS24690	4757199..4758140	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ECO111_RS24695	4758204..4759112	-	909	WP_001162704.1	alpha/beta hydrolase	-
ECO111_RS24700	4759341..4759652	+	312	WP_000897305.1	hypothetical protein	Toxin
ECO111_RS24705	4759653..4759943	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ECO111_RS24710	4760548..4760766	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
ECO111_RS24715	4760985..4761227	+	243	WP_001086388.1	hypothetical protein	-
ECO111_RS24725	4761558..4762487	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
ECO111_RS24730	4762484..4763119	-	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
ECO111_RS24735	4763116..4764018	-	903	WP_000331369.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T24366 WP_000897305.1 NC_013364:4759341-4759652 [Escherichia coli O111:H- str. 11128]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T24366 NC_013364:4759341-4759652 [Escherichia coli O111:H- str. 11128]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT24366 WP_000356397.1 NC_013364:4759653-4759943 [Escherichia coli O111:H- str. 11128]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT24366 NC_013364:4759653-4759943 [Escherichia coli O111:H- str. 11128]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7