Detailed information of TA system TA024362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3406287..3407014
• Replicon: chromosome
• Accession: NC_013364
• Organism: Escherichia coli O111:H- str. 11128

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: ECO111_RS17980
• Protein ID: WP_000547559.1
• Coordinates: 3406703..3407014 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECO111_RS17975
• Protein ID: WP_000126294.1
• Coordinates: 3406287..3406706 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO111_RS17955	3401337..3401864	-	528	WP_001078781.1	electron transport protein HydN	-
ECO111_RS17960	3402013..3403023	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
ECO111_RS17965	3403283..3404740	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
ECO111_RS17970	3404749..3406173	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
ECO111_RS17975	3406287..3406706	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
ECO111_RS17980	3406703..3407014	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
ECO111_RS17985	3407188..3407931	-	744	WP_001369451.1	hypothetical protein	-
ECO111_RS17990	3407974..3408444	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
ECO111_RS17995	3408437..3408847	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
ECO111_RS18000	3408844..3409611	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
ECO111_RS18005	3409611..3410153	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
ECO111_RS18010	3410163..3411872	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T24362 WP_000547559.1 NC_013364:c3407014-3406703 [Escherichia coli O111:H- str. 11128]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T24362 NC_013364:c3407014-3406703 [Escherichia coli O111:H- str. 11128]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT24362 WP_000126294.1 NC_013364:c3406706-3406287 [Escherichia coli O111:H- str. 11128]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT24362 NC_013364:c3406706-3406287 [Escherichia coli O111:H- str. 11128]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163