Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2667506..2668008 Replicon chromosome
Accession NC_013364
Organism Escherichia coli O111:H- str. 11128

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECO111_RS14340 Protein ID WP_000767829.1
Coordinates 2667506..2667760 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECO111_RS14345 Protein ID WP_001259255.1
Coordinates 2667757..2668008 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO111_RS14315 2662521..2662748 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ECO111_RS14320 2662762..2663820 - 1059 WP_000492340.1 transport protein YeeE -
ECO111_RS14325 2663999..2665357 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECO111_RS33120 2665347..2665409 - 63 WP_010723108.1 membrane protein YoeI -
ECO111_RS14330 2665624..2666553 - 930 WP_000803331.1 LysR family transcriptional regulator -
ECO111_RS14335 2666599..2667423 - 825 WP_000754755.1 SDR family oxidoreductase -
ECO111_RS14340 2667506..2667760 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECO111_RS14345 2667757..2668008 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECO111_RS31590 2668292..2668342 + 51 WP_001364200.1 his operon leader peptide -
ECO111_RS14350 2668488..2669387 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECO111_RS14355 2669393..2670697 + 1305 WP_001345258.1 histidinol dehydrogenase -
ECO111_RS14360 2670694..2671773 + 1080 WP_000102292.1 histidinol-phosphate transaminase -
ECO111_RS14365 2671770..2672837 + 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T24360 WP_000767829.1 NC_013364:c2667760-2667506 [Escherichia coli O111:H- str. 11128]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T24360 NC_013364:c2667760-2667506 [Escherichia coli O111:H- str. 11128]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT24360 WP_001259255.1 NC_013364:c2668008-2667757 [Escherichia coli O111:H- str. 11128]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT24360 NC_013364:c2668008-2667757 [Escherichia coli O111:H- str. 11128]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References