Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2196897..2197765 Replicon chromosome
Accession NZ_CP096840
Organism Mycobacterium tuberculosis variant bovis strain Mb2269

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag M1780_RS10255 Protein ID WP_010886136.1
Coordinates 2196897..2197274 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag M1780_RS10260 Protein ID WP_003409886.1
Coordinates 2197316..2197765 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1780_RS10205 (M1780_10205) 2192686..2193138 - 453 WP_010950636.1 lipoprotein -
M1780_RS10210 (M1780_10210) 2193202..2193603 + 402 WP_003409869.1 hypothetical protein -
M1780_RS10215 (M1780_10215) 2193596..2193778 - 183 WP_003409870.1 hypothetical protein -
M1780_RS10220 (M1780_10220) 2193892..2194242 - 351 WP_003409871.1 hypothetical protein -
M1780_RS10225 (M1780_10225) 2194253..2195155 - 903 WP_003409874.1 hypothetical protein -
M1780_RS10230 (M1780_10230) 2195176..2195367 - 192 WP_003409876.1 hypothetical protein -
M1780_RS10235 (M1780_10235) 2195368..2195664 - 297 WP_003409877.1 hypothetical protein -
M1780_RS10240 (M1780_10240) 2195904..2196119 + 216 WP_003409878.1 antitoxin -
M1780_RS10245 (M1780_10245) 2196116..2196427 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
M1780_RS10250 (M1780_10250) 2196401..2196922 - 522 WP_010950637.1 hypothetical protein -
M1780_RS10255 (M1780_10255) 2196897..2197274 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
M1780_RS10260 (M1780_10260) 2197316..2197765 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
M1780_RS10265 (M1780_10265) 2197762..2198307 + 546 WP_003409891.1 SecB-like chaperone -
M1780_RS10270 (M1780_10270) 2198196..2198810 - 615 WP_003901296.1 hypothetical protein -
M1780_RS10275 (M1780_10275) 2198859..2199155 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
M1780_RS10280 (M1780_10280) 2199152..2199403 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
M1780_RS10285 (M1780_10285) 2199390..2199884 + 495 WP_003899099.1 hypothetical protein -
M1780_RS10290 (M1780_10290) 2200044..2200451 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
M1780_RS10295 (M1780_10295) 2200455..2200727 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
M1780_RS10300 (M1780_10300) 2200760..2201980 - 1221 WP_273610366.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T243584 WP_010886136.1 NZ_CP096840:2196897-2197274 [Mycobacterium tuberculosis variant bovis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T243584 NZ_OX419553:c1323882-1323136 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTATATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTAGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAGCCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT243584 WP_003409886.1 NZ_CP096840:2197316-2197765 [Mycobacterium tuberculosis variant bovis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT243584 NZ_OX419553:c1323136-1322966 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References