Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3511905..3512575 Replicon chromosome
Accession NZ_CP096839
Organism Mycobacterium tuberculosis variant bovis strain Mb2487

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag M1779_RS16580 Protein ID WP_003899954.1
Coordinates 3511905..3512249 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag M1779_RS16585 Protein ID WP_003899955.1
Coordinates 3512246..3512575 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1779_RS16545 (M1779_16545) 3506978..3507838 + 861 WP_003416628.1 alpha/beta hydrolase -
M1779_RS16550 (M1779_16550) 3507813..3508328 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
M1779_RS16555 (M1779_16555) 3508344..3508555 + 212 Protein_3268 (R)-hydratase -
M1779_RS16560 (M1779_16560) 3508568..3508861 + 294 WP_003416635.1 hypothetical protein -
M1779_RS16565 (M1779_16565) 3509149..3510438 + 1290 WP_003416640.1 ATP-binding protein -
M1779_RS16570 (M1779_16570) 3510785..3511219 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
M1779_RS16575 (M1779_16575) 3511222..3511674 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
M1779_RS16580 (M1779_16580) 3511905..3512249 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M1779_RS16585 (M1779_16585) 3512246..3512575 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
M1779_RS16590 (M1779_16590) 3513113..3513460 + 348 WP_003899956.1 DUF2384 domain-containing protein -
M1779_RS16595 (M1779_16595) 3513457..3514077 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
M1779_RS16600 (M1779_16600) 3514237..3515502 - 1266 WP_003909837.1 hypothetical protein -
M1779_RS16605 (M1779_16605) 3515670..3515879 + 210 WP_003416778.1 hypothetical protein -
M1779_RS16610 (M1779_16610) 3516126..3517160 - 1035 WP_044292644.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T243548 WP_003899954.1 NZ_CP096839:3511905-3512249 [Mycobacterium tuberculosis variant bovis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T243548 NZ_OX359182:3128513-3129046 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT243548 WP_003899955.1 NZ_CP096839:3512246-3512575 [Mycobacterium tuberculosis variant bovis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT243548 NZ_OX359182:3128236-3128502 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References