Detailed information of TA system TA243467

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 72641..72881
• Replicon: plasmid pPM4-5-103k
• Accession: NZ_CP096835
• Organism: Escherichia coli strain PM4-5

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MYF75_RS25280
• Protein ID: WP_001372321.1
• Coordinates: 72641..72766 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	72843..72881 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYF75_RS25235 (67688)	67688..67915	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
MYF75_RS25240 (68011)	68011..68697	-	687	WP_015059009.1	PAS domain-containing protein	-
MYF75_RS25245 (68888)	68888..69271	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MYF75_RS25250 (69548)	69548..70195	+	648	WP_148726238.1	transglycosylase SLT domain-containing protein	-
MYF75_RS25255 (70492)	70492..71313	-	822	WP_033804334.1	DUF932 domain-containing protein	-
MYF75_RS25260 (71432)	71432..71719	-	288	WP_000107535.1	hypothetical protein	-
MYF75_RS25265 (71744)	71744..71950	-	207	WP_033804333.1	hypothetical protein	-
MYF75_RS25270 (72020)	72020..72193	+	174	Protein_74	hypothetical protein	-
MYF75_RS25275 (72191)	72191..72421	-	231	WP_071845785.1	hypothetical protein	-
MYF75_RS25280 (72641)	72641..72766	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
MYF75_RS25285 (72708)	72708..72857	-	150	Protein_77	plasmid maintenance protein Mok	-
- (72843)	72843..72881	-	39	NuclAT_1	-	Antitoxin
- (72843)	72843..72881	-	39	NuclAT_1	-	Antitoxin
- (72843)	72843..72881	-	39	NuclAT_1	-	Antitoxin
- (72843)	72843..72881	-	39	NuclAT_1	-	Antitoxin
- (74323)	74323..74513	-	191	NuclAT_0	-	-
- (74323)	74323..74513	-	191	NuclAT_0	-	-
- (74323)	74323..74513	-	191	NuclAT_0	-	-
- (74323)	74323..74513	-	191	NuclAT_0	-	-
MYF75_RS25295 (74325)	74325..74513	+	189	WP_063073474.1	hypothetical protein	-
MYF75_RS25300 (74482)	74482..75244	-	763	Protein_80	plasmid SOS inhibition protein A	-
MYF75_RS25305 (75241)	75241..75675	-	435	WP_000845907.1	conjugation system SOS inhibitor PsiB	-
MYF75_RS25310 (75730)	75730..77688	-	1959	WP_063072993.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..103920	103920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T243467 WP_001372321.1 NZ_CP096835:c72766-72641 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T243467 NZ_OX352324:c4196408-4196127 [Pseudomonas sp. MM221]
ATGACCTATAAGCTTGAATTCCTGCCTTCGGCGCTGAAGGAGTGGGGCAAACTGGGCCACACCGTGCGCGAACAGATCAA
GAAAAAACTTGGCGAACGTTTGCAATCGCCCAAGGTACAGGCCGATGCACTCCGCGACCTGGCGAACCACTATAAAATCA
AGCTCAAGGCCTCCGGATATCGCCTGGTCTATCGGGTCGAGGACGAGCGTATCGTTGTGGTGGTGGTATCGGTTGGAAAG
CGCGAGCGCAGCGAGGTCTATCGAGCCGCTCAGAAGCGCTAG

Antitoxin

Nucleotide

• Length: 39 bp

>AT243467 NZ_CP096835:c72881-72843 [Escherichia coli]
CATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin