Detailed information of TA system TA243461

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3816366..3816612
• Replicon: chromosome
• Accession: NZ_CP096834
• Organism: Escherichia coli strain PM4-5

Toxin (Protein)

• Gene name: hokX
• Uniprot ID: S1PD89
• Locus tag: MYF75_RS20040
• Protein ID: WP_000956458.1
• Coordinates: 3816460..3816612 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	sokX
Locus tag	-
Coordinates	3816366..3816420 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYF75_RS20025	3811876..3813675	+	1800	WP_000211906.1	NADPH-dependent assimilatory sulfite reductase flavoprotein subunit	-
MYF75_RS20030	3813675..3815387	+	1713	WP_001290706.1	assimilatory sulfite reductase (NADPH) hemoprotein subunit	-
MYF75_RS20035	3815461..3816195	+	735	WP_000039843.1	phosphoadenosine phosphosulfate reductase	-
-	3816366..3816420	-	55	-	-	Antitoxin
MYF75_RS20040	3816460..3816612	+	153	WP_000956458.1	Hok/Gef family protein	Toxin
MYF75_RS20045	3816806..3819505	+	2700	WP_001233961.1	CRISPR-associated helicase/endonuclease Cas3	-
MYF75_RS20050	3819603..3821165	+	1563	WP_001084093.1	type I-E CRISPR-associated protein Cse1/CasA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (5-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5558.77 Da
• Isoelectric Point: 7.6757

>T243461 WP_000956458.1 NZ_CP096834:3816460-3816612 [Escherichia coli]
MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVLAYESKK

Nucleotide

• Length: 153 bp

>T243461 NZ_OX352322:5683586-5684053 [Pseudomonas sp. MM223]
ATGAGTGATGCGAGCAGGAAGCCTCTGGTTATTCATGGATGGACTGTAATTGCCCACCCGCTGTTCCTGGCCAAACTGGA
CGCGCTCAGCGCTCAAGTGCAGGCTCAGATGCACAAGGACCCAACGGGTTACACGAGCAGAAATGCCTATAAAAGGCTGG
CGGCCATCAAGCGACTGGCCTTCGATGTTATCCCTCAAGACCCGACCAAACCGGAATACCGGCAGGGGGCCACACTGGGT
GGTGACCACAAGCACTGGTTCAGGGCAAAGTTCTTCCAGCAGTACCGGTTGTTCTTTCGCTACCACACCCCCAGCAGGAT
CATTGTGCTGGCCTGGGTCAATGACGAGTCGAGCAAGCGGGCCTACGAAAGTAGCGACGATGCTTACAAGGTCTTTCAGA
AGATGTTGCATACCGGCCACCCGCCGGACGATTGGGAGCAGCTACTGCGAGAAGCTGCAGCCGAATGA

Antitoxin

Nucleotide

• Length: 55 bp

>AT243461 NZ_CP096834:c3816420-3816366 [Escherichia coli]
GTTTGGGTTCGAACGTAAGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGT

Structures

Toxin

• AlphaFold DB: A0A4P7TJ83

Antitoxin