Detailed information of TA system TA024346

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1744135..1744506
• Replicon: chromosome
• Accession: NC_013364
• Organism: Escherichia coli O111:H- str. 11128

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: ECO111_RS09145
• Protein ID: WP_001443846.1
• Coordinates: 1744357..1744506 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1744135..1744313 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO111_RS34050	1739887..1740060	+	174	WP_001296046.1	hypothetical protein	-
ECO111_RS09120	1740090..1741463	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
ECO111_RS09125	1741592..1742527	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
ECO111_RS31105	1742579..1743814	-	1236	WP_000040839.1	site-specific integrase	-
ECO111_RS09135	1743816..1744031	-	216	WP_000079604.1	excisionase XisR	-
ECO111_RS09140	1744131..1744319	-	189	WP_001302840.1	DUF1187 family protein	-
-	1744135..1744313	+	179	NuclAT_0	-	Antitoxin
-	1744135..1744313	+	179	NuclAT_0	-	Antitoxin
-	1744135..1744313	+	179	NuclAT_0	-	Antitoxin
-	1744135..1744313	+	179	NuclAT_0	-	Antitoxin
ECO111_RS09145	1744357..1744506	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
ECO111_RS31110	1744562..1744777	-	216	Protein_1720	recombinase RecT	-
ECO111_RS09160	1746107..1746283	+	177	WP_000285960.1	phage antirepressor KilAC domain-containing protein	-
ECO111_RS33730	1746378..1746509	+	132	WP_001280923.1	hypothetical protein	-
ECO111_RS33740	1746732..1746917	+	186	WP_012817877.1	Rz1 family lipoprotein	-
ECO111_RS09180	1747318..1747644	+	327	WP_000828070.1	TonB family protein	-
ECO111_RS09185	1747776..1747976	-	201	WP_000095744.1	YlcI/YnfO family protein	-
ECO111_RS09190	1748018..1748383	+	366	WP_000829192.1	HNH endonuclease	-
ECO111_RS09195	1748672..1749235	+	564	WP_000958380.1	terminase small subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1735320..1770952	35632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T24346 WP_001443846.1 NC_013364:c1744506-1744357 [Escherichia coli O111:H- str. 11128]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T24346 NC_013364:c1744506-1744357 [Escherichia coli O111:H- str. 11128]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT24346 NC_013364:1744135-1744313 [Escherichia coli O111:H- str. 11128]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin