243458

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3363340..3364139 Replicon chromosome
Accession NZ_CP096834
Organism Escherichia coli strain PM4-5

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag MYF75_RS17860 Protein ID WP_000347267.1
Coordinates 3363340..3363804 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MYF75_RS17865 Protein ID WP_001307405.1
Coordinates 3363804..3364139 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF75_RS17830 (3358341) 3358341..3358775 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MYF75_RS17835 (3358793) 3358793..3359671 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
MYF75_RS17840 (3359661) 3359661..3360440 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
MYF75_RS17845 (3360451) 3360451..3360924 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MYF75_RS17850 (3360947) 3360947..3362227 - 1281 WP_000681947.1 tagatose-bisphosphate aldolase subunit KbaZ -
MYF75_RS17855 (3362476) 3362476..3363285 + 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
MYF75_RS17860 (3363340) 3363340..3363804 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MYF75_RS17865 (3363804) 3363804..3364139 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MYF75_RS17870 (3364288) 3364288..3365859 - 1572 WP_001273741.1 galactarate dehydratase -
MYF75_RS17875 (3366234) 3366234..3367568 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MYF75_RS17880 (3367584) 3367584..3368354 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T243458 WP_000347267.1 NZ_CP096834:c3363804-3363340 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T243458 NZ_OX352322:5041685-5041987 [Pseudomonas sp. MM223]
ATGAAGAAAATCGAATCCAGCAGTTTCAGACATTGGGTGACCGGTCTCCGGGACGTTAACGCCAGGGCCCGAATCATTTC
TCGGATCAACCGGTTGATGGAGGGTCTGCCTGGCGACGTATCACCGGTTGGCCAAGGCATTAGTGAACTCAGAATTCACT
ATGGGCCTGGCTACCGGGTTTACTTCCATCAGGTGGGCAGCACATTCGTCATTCTGCTTTGCGGCGGTGAAAAAAGTAGC
CAACAGCGAGACATCAAGACAGCCCACCAGATTCTGCGTTCGTGGAGGATACAAAATGACTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT243458 WP_001307405.1 NZ_CP096834:c3364139-3363804 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT243458 NZ_OX352322:5041980-5042273 [Pseudomonas sp. MM223]
ATGACTGAGACCTTCTCTGAATTCGATCCCGCCGAGTACCTCACTACCCCTGAGGCAATCGCGCTGTTCATGACTGACGC
CCTTGAGACCGGTGATGCCGGCTATGTCGCCAAGGCCGTAGGTGTGGTGGCACGTGCAAAAGGCATGAGCGAACTGGCGA
AAGAGACCGGGCTGTCACGAGAGCAGCTTTACAGGTCTTTCAGCGAGCATGGCAATCCGACCCTGAAGTCTTTTCTGACC
GTGATGAAAGCACTGGGTATAGACATGACGGCGCGCCCCCACCTCGCTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References