Detailed information of TA system TA243455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2568120..2568722
• Replicon: chromosome
• Accession: NZ_CP096834
• Organism: Escherichia coli strain PM4-5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: MYF75_RS13980
• Protein ID: WP_000897305.1
• Coordinates: 2568411..2568722 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MYF75_RS13975
• Protein ID: WP_000356397.1
• Coordinates: 2568120..2568410 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYF75_RS13950 (2563667)	2563667..2564302	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
MYF75_RS13955 (2564299)	2564299..2565228	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
MYF75_RS13960 (2565627)	2565627..2566607	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
MYF75_RS13965 (2566836)	2566836..2567078	-	243	WP_001086388.1	protein YiiF	-
MYF75_RS13970 (2567297)	2567297..2567515	-	219	WP_001295676.1	CopG family transcriptional regulator	-
MYF75_RS13975 (2568120)	2568120..2568410	-	291	WP_000356397.1	NadS family protein	Antitoxin
MYF75_RS13980 (2568411)	2568411..2568722	-	312	WP_000897305.1	hypothetical protein	Toxin
MYF75_RS13985 (2568951)	2568951..2569859	+	909	WP_001162704.1	alpha/beta hydrolase	-
MYF75_RS13990 (2569923)	2569923..2570864	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
MYF75_RS13995 (2570909)	2570909..2571346	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
MYF75_RS14000 (2571343)	2571343..2572215	-	873	WP_000920762.1	virulence factor BrkB family protein	-
MYF75_RS14005 (2572209)	2572209..2572808	-	600	WP_001295269.1	glucose-1-phosphatase	-
MYF75_RS14010 (2572907)	2572907..2573692	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2565627..2566607	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T243455 WP_000897305.1 NZ_CP096834:c2568722-2568411 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T243455 NZ_OX352322:c3568966-3568718 [Pseudomonas sp. MM223]
ATGTCCAAGCAAGAAAAGCTGCTCGCGAAGCTGCTGAACAAGCAAGCAGGATTCACTTGGCCGGAGCTGGTTACGCTGCT
GGGTGGGCTTGGATACCGCCAGATCGAGGGTGACGGCAGCCGGGTAAAATTCGACAACGGCCACCCGCAGGCGATGATCA
ACCTGCACAAGCCGCACCCCGGCAATGAGCTGAAGGCCTACGTTAAACGCCAGGTGATAGAACACCTGAAGGCAGGAGGA
TTGATCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT243455 WP_000356397.1 NZ_CP096834:c2568410-2568120 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT243455 NZ_OX352322:c3568718-3568377 [Pseudomonas sp. MM223]
ATGAGCACCATGTTGCAGCACCGAGGCTACTACGGCTCCATTGAGGCCAGCCCCGAAGACAATTGCCTGTTCGGCAAGCT
CCAGTTCATCCGCGCCCTTGTCAGCTATGAGGGCGAGACGGTTGCCGAGCTGACCCAGGCGTTCCGCGATGCAGTGGACG
ACTACCTGGATACCTGCGCATCACTCGGGCAGGAGCCGGAGATTCCGTGCAAGGGGTCCTTCAACGTGAGGGTAGGGCAC
GACCTGCACCTGGCAGCCAGCGTGGCGGCTACCCAGCAAAACATTTCTTTGAATGACCTCACCCGTAAAGCGCTCAGCGA
ATACCTGGGCCATCACGCATAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7