243449

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1746769..1747310 Replicon chromosome
Accession NZ_CP096834
Organism Escherichia coli strain PM4-5

Toxin (Protein)


Gene name yafQ Uniprot ID A7ZHY5
Locus tag MYF75_RS10220 Protein ID WP_012000916.1
Coordinates 1747032..1747310 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag MYF75_RS10215 Protein ID WP_000729704.1
Coordinates 1746769..1747029 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF75_RS10190 (1742552) 1742552..1743337 - 786 WP_000207557.1 putative lateral flagellar export/assembly protein LafU -
MYF75_RS10195 (1743309) 1743309..1745021 + 1713 Protein_1687 flagellar biosynthesis protein FlhA -
MYF75_RS10200 (1745126) 1745126..1745404 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
MYF75_RS10205 (1745397) 1745397..1745753 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
MYF75_RS10210 (1745810) 1745810..1746583 - 774 WP_000543899.1 C40 family peptidase -
MYF75_RS10215 (1746769) 1746769..1747029 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
MYF75_RS10220 (1747032) 1747032..1747310 + 279 WP_012000916.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
MYF75_RS10225 (1747466) 1747466..1748206 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
MYF75_RS10230 (1748177) 1748177..1748944 - 768 WP_000333380.1 class II glutamine amidotransferase -
MYF75_RS10235 (1749150) 1749150..1749728 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T243449 WP_012000916.1 NZ_CP096834:1747032-1747310 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T243449 NZ_OX352320:c698153-697869 [Rheinheimera sp. MM224]
ATGATTTACTGGGAAGAGGAGGCGCTGAACGATAGGGAAAAAATTTTTGAATACCTTTATGCAGTAAACCCTTTAGCCGC
CGAAAGAACCGATGAACTTATCGAAGCCAAAGTCGAAAATCTATTGGATCACCCGTTAATGGGTATCCAGCGCGCTTCGG
TCCGTGGTCGTTTGCTGATTATTCCTGAAGTATCCATGATTGTTTCTTATTGGATTGATGGTTCAACTATCCGGGTTATG
CGGGTTTTGCATCAAAAGCAGCAGTTTCCCGGGCTAGAAAGATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT243449 WP_000729704.1 NZ_CP096834:1746769-1747029 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT243449 NZ_OX352320:c698434-698150 [Rheinheimera sp. MM224]
ATGGAAACCAGAATTCAGTTTCGTGTTGATGAAGAAACCAAACGCCTTGCTCAGCTGATGGCTGAAAGTCAGGGGCGAAC
TTTAAGTGATGCCTGCCGCCAACTCACTGAGCAACTCGCAGCTGAGCAACGAAAAGTCAGCGCCCATGATCAATGGCTCA
CAGTGCAAGTAAATGCTGCCTTTGAAAAACTGGATACAGGTAACGCTAAGTTCGTCAGTCACGAACAAGCGAAAATCAGC
ATGGCAGAACGCAAAGCTAAAATTCGCAGTAGAGCTAACGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A7ZHY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6Z6

References