Detailed information of TA system TA243428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5524955..5525550
• Replicon: chromosome
• Accession: NZ_CP096822
• Organism: Pseudomonas aeruginosa strain NY8709

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: M1V28_RS25530
• Protein ID: WP_003117425.1
• Coordinates: 5525272..5525550 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M1V28_RS25525
• Protein ID: WP_003113527.1
• Coordinates: 5524955..5525260 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1V28_RS25490 (M1V28_25450)	5520095..5520943	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
M1V28_RS25500 (M1V28_25460)	5521110..5522051	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
M1V28_RS25505 (M1V28_25465)	5522168..5522782	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
M1V28_RS25510 (M1V28_25470)	5522824..5523408	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
M1V28_RS25515 (M1V28_25475)	5523449..5524549	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
M1V28_RS25525 (M1V28_25485)	5524955..5525260	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
M1V28_RS25530 (M1V28_25490)	5525272..5525550	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M1V28_RS25535 (M1V28_25495)	5525603..5525731	-	129	Protein_5044	integrase	-
M1V28_RS25540 (M1V28_25500)	5525879..5528107	+	2229	WP_004352671.1	TonB-dependent receptor	-
M1V28_RS25545 (M1V28_25505)	5528177..5528824	-	648	WP_003095021.1	carbonate dehydratase	-
M1V28_RS25550 (M1V28_25510)	5528886..5530124	-	1239	WP_023123442.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T243428 WP_003117425.1 NZ_CP096822:c5525550-5525272 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T243428 NZ_OX337252:c5331433-5331050 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT243428 WP_003113527.1 NZ_CP096822:c5525260-5524955 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT243428 NZ_OX337252:c5331675-5331433 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5