Detailed information of TA system TA243426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4871285..4871966
• Replicon: chromosome
• Accession: NZ_CP096822
• Organism: Pseudomonas aeruginosa strain NY8709

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: M1V28_RS22440
• Protein ID: WP_015503432.1
• Coordinates: 4871601..4871966 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M1V28_RS22435
• Protein ID: WP_016561576.1
• Coordinates: 4871285..4871608 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1V28_RS22400 (M1V28_22360)	4866906..4867784	+	879	WP_003109435.1	hypothetical protein	-
M1V28_RS22410 (M1V28_22370)	4868296..4869162	-	867	WP_023081971.1	hypothetical protein	-
M1V28_RS22415 (M1V28_22375)	4869192..4869443	-	252	WP_124136459.1	hypothetical protein	-
M1V28_RS22420 (M1V28_22380)	4869660..4870663	-	1004	Protein_4435	tyrosine-type recombinase/integrase	-
M1V28_RS22425 (M1V28_22385)	4870663..4871055	-	393	Protein_4436	hypothetical protein	-
M1V28_RS22430 (M1V28_22390)	4871050..4871169	+	120	Protein_4437	IS5/IS1182 family transposase	-
M1V28_RS22435 (M1V28_22395)	4871285..4871608	-	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
M1V28_RS22440 (M1V28_22400)	4871601..4871966	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M1V28_RS22445 (M1V28_22405)	4872230..4872472	-	243	WP_043884955.1	hypothetical protein	-
M1V28_RS22450 (M1V28_22410)	4872679..4872951	+	273	WP_003085667.1	hypothetical protein	-
M1V28_RS22455 (M1V28_22415)	4872970..4873395	-	426	WP_003163196.1	VOC family protein	-
M1V28_RS22460 (M1V28_22420)	4873496..4874380	+	885	WP_019725847.1	LysR substrate-binding domain-containing protein	-
M1V28_RS22465 (M1V28_22425)	4874353..4875306	-	954	WP_003085661.1	LysR substrate-binding domain-containing protein	-
M1V28_RS22470 (M1V28_22430)	4875527..4875961	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T243426 WP_015503432.1 NZ_CP096822:c4871966-4871601 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T243426 NZ_OX337252:c4842844-4842560 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT243426 WP_016561576.1 NZ_CP096822:c4871608-4871285 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT243426 NZ_OX337252:c4843076-4842834 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA