Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 321174..321769 Replicon chromosome
Accession NZ_CP096813
Organism Pseudomonas aeruginosa strain 8D

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag M1Q10_RS01505 Protein ID WP_003113526.1
Coordinates 321491..321769 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M1Q10_RS01500 Protein ID WP_003113527.1
Coordinates 321174..321479 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1Q10_RS01475 (M1Q10_01475) 317127..317417 - 291 WP_223683635.1 DUF5447 family protein -
M1Q10_RS01480 (M1Q10_01480) 317593..317865 - 273 WP_223683634.1 DNA-binding protein -
M1Q10_RS01485 (M1Q10_01485) 317975..318241 + 267 WP_023088595.1 hypothetical protein -
M1Q10_RS01490 (M1Q10_01490) 318297..318632 + 336 WP_174515448.1 hypothetical protein -
M1Q10_RS01495 (M1Q10_01495) 318655..320169 - 1515 WP_223683633.1 ATP-binding protein -
M1Q10_RS01500 (M1Q10_01500) 321174..321479 - 306 WP_003113527.1 HigA family addiction module antitoxin Antitoxin
M1Q10_RS01505 (M1Q10_01505) 321491..321769 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M1Q10_RS01510 (M1Q10_01510) 321822..321950 - 129 Protein_293 integrase -
M1Q10_RS01515 (M1Q10_01515) 322098..324326 + 2229 WP_003099265.1 TonB-dependent receptor -
M1Q10_RS01520 (M1Q10_01520) 324396..325043 - 648 WP_003095021.1 carbonate dehydratase -
M1Q10_RS01525 (M1Q10_01525) 325105..326343 - 1239 WP_010793756.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T243384 WP_003113526.1 NZ_CP096813:c321769-321491 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T243384 NZ_OX030728:c385425-385318 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11247.81 Da        Isoelectric Point: 5.0747

>AT243384 WP_003113527.1 NZ_CP096813:c321479-321174 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp

>AT243384 NZ_OX030728:385478-385539 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure

References