Detailed information of TA system TA243369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3641027..3641673
• Replicon: chromosome
• Accession: NZ_CP096810
• Organism: Klebsiella pneumoniae strain Bio19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MOV22_RS17565
• Protein ID: WP_087775076.1
• Coordinates: 3641027..3641374 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A231WWU8
• Locus tag: MOV22_RS17570
• Protein ID: WP_004174017.1
• Coordinates: 3641374..3641673 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOV22_RS17555 (3636953)	3636953..3638386	+	1434	WP_002920564.1	glycogen synthase GlgA	-
MOV22_RS17560 (3638404)	3638404..3640851	+	2448	WP_002920561.1	glycogen phosphorylase	-
MOV22_RS17565 (3641027)	3641027..3641374	+	348	WP_087775076.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MOV22_RS17570 (3641374)	3641374..3641673	+	300	WP_004174017.1	XRE family transcriptional regulator	Antitoxin
MOV22_RS17575 (3641736)	3641736..3643244	-	1509	WP_087775074.1	glycerol-3-phosphate dehydrogenase	-
MOV22_RS17580 (3643449)	3643449..3643778	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
MOV22_RS17585 (3643829)	3643829..3644659	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
MOV22_RS17590 (3644709)	3644709..3645467	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13508.51 Da
• Isoelectric Point: 6.2327

>T243369 WP_087775076.1 NZ_CP096810:3641027-3641374 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDSLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T243369 NZ_OX030701:3323178-3323528 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10844.64 Da
• Isoelectric Point: 10.1464

>AT243369 WP_004174017.1 NZ_CP096810:3641374-3641673 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT243369 NZ_OX030701:3322933-3323184 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A231WWU8