Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 3614840..3615426 Replicon chromosome
Accession NZ_CP096810
Organism Klebsiella pneumoniae strain Bio19

Toxin (Protein)


Gene name doc Uniprot ID W9BMV7
Locus tag MOV22_RS17455 Protein ID WP_004185990.1
Coordinates 3615058..3615426 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag MOV22_RS17450 Protein ID WP_004174006.1
Coordinates 3614840..3615061 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOV22_RS17430 (3610997) 3610997..3611923 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MOV22_RS17435 (3611920) 3611920..3613197 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
MOV22_RS17440 (3613194) 3613194..3613961 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
MOV22_RS17445 (3613963) 3613963..3614676 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
MOV22_RS17450 (3614840) 3614840..3615061 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MOV22_RS17455 (3615058) 3615058..3615426 + 369 WP_004185990.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MOV22_RS17460 (3615699) 3615699..3617015 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
MOV22_RS17465 (3617122) 3617122..3618009 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
MOV22_RS17470 (3618006) 3618006..3618851 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
MOV22_RS17475 (3618853) 3618853..3619923 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3613194..3620660 7466


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13469.85 Da        Isoelectric Point: 8.0667

>T243368 WP_004185990.1 NZ_CP096810:3615058-3615426 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVCRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T243368 NZ_OX030701:3220254-3220427 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT243368 WP_004174006.1 NZ_CP096810:3614840-3615061 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT243368 NZ_OX030701:3219930-3220226 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BMV7

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References