243362

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2451206..2451803 Replicon chromosome
Accession NZ_CP096810
Organism Klebsiella pneumoniae strain Bio19

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag MOV22_RS11905 Protein ID WP_004142563.1
Coordinates 2451206..2451523 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag MOV22_RS11910 Protein ID WP_004142561.1
Coordinates 2451516..2451803 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOV22_RS11890 (2446908) 2446908..2448335 - 1428 WP_064148261.1 MFS transporter -
MOV22_RS11895 (2448444) 2448444..2449310 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
MOV22_RS11900 (2449980) 2449980..2451032 + 1053 WP_117094318.1 DUF2157 domain-containing protein -
MOV22_RS11905 (2451206) 2451206..2451523 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MOV22_RS11910 (2451516) 2451516..2451803 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
MOV22_RS11915 (2452068) 2452068..2452721 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
MOV22_RS11920 (2452841) 2452841..2453209 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
MOV22_RS11925 (2453209) 2453209..2453790 - 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
MOV22_RS11930 (2453970) 2453970..2455085 + 1116 WP_012737592.1 MBL fold metallo-hydrolase -
MOV22_RS11935 (2455116) 2455116..2455457 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
MOV22_RS11940 (2455475) 2455475..2455723 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T243362 WP_004142563.1 NZ_CP096810:2451206-2451523 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T243362 NZ_OX030701:c2038820-2038506 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT243362 WP_004142561.1 NZ_CP096810:2451516-2451803 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT243362 NZ_OX030701:c2038509-2038093 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References