Detailed information of TA system TA024335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4698590..4699192
• Replicon: chromosome
• Accession: NC_013361
• Organism: Escherichia coli O26:H11 str. 11368

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: ECO26_RS24690
• Protein ID: WP_000897305.1
• Coordinates: 4698881..4699192 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECO26_RS24685
• Protein ID: WP_000356397.1
• Coordinates: 4698590..4698880 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO26_RS24655	4694136..4694771	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
ECO26_RS24660	4694768..4695697	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
ECO26_RS24665	4696097..4697077	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
ECO26_RS24675	4697306..4697548	-	243	WP_001086390.1	hypothetical protein	-
ECO26_RS24680	4697767..4697985	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
ECO26_RS24685	4698590..4698880	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ECO26_RS24690	4698881..4699192	-	312	WP_000897305.1	hypothetical protein	Toxin
ECO26_RS24695	4699421..4700329	+	909	WP_001162704.1	alpha/beta hydrolase	-
ECO26_RS24700	4700393..4701334	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ECO26_RS24705	4701379..4701816	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ECO26_RS24710	4701813..4702685	-	873	WP_000920762.1	virulence factor BrkB family protein	-
ECO26_RS24715	4702679..4703278	-	600	WP_001315111.1	glucose-1-phosphatase	-
ECO26_RS24720	4703377..4704162	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4696097..4697077	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T24335 WP_000897305.1 NC_013361:c4699192-4698881 [Escherichia coli O26:H11 str. 11368]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T24335 NC_013361:c4699192-4698881 [Escherichia coli O26:H11 str. 11368]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT24335 WP_000356397.1 NC_013361:c4698880-4698590 [Escherichia coli O26:H11 str. 11368]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT24335 NC_013361:c4698880-4698590 [Escherichia coli O26:H11 str. 11368]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7