Detailed information of TA system TA243329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2769..3420
• Replicon: plasmid p5656
• Accession: NZ_CP096758
• Organism: Acinetobacter baumannii strain 5656

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: M1S36_RS19170
• Protein ID: WP_000269904.1
• Coordinates: 2769..3125 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M1S36_RS19175
• Protein ID: WP_001129974.1
• Coordinates: 3118..3420 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1S36_RS19155 (M1S36_19155)	163..1098	+	936	WP_001292329.1	replication initiation protein RepM	-
M1S36_RS19160 (M1S36_19160)	1176..1694	+	519	WP_000839337.1	plasmid replication DNA-binding protein	-
M1S36_RS19165 (M1S36_19165)	1904..2203	+	300	WP_000358348.1	hypothetical protein	-
M1S36_RS19170 (M1S36_19170)	2769..3125	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M1S36_RS19175 (M1S36_19175)	3118..3420	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
M1S36_RS19180 (M1S36_19180)	3413..3622	+	210	WP_000069474.1	hypothetical protein	-
M1S36_RS19185 (M1S36_19185)	3948..4409	-	462	WP_000761346.1	DIP1984 family protein	-
M1S36_RS19190 (M1S36_19190)	4714..7125	-	2412	WP_000932953.1	TonB-dependent receptor ZnuD2	-
M1S36_RS19195 (M1S36_19195)	7253..7567	-	315	WP_001983304.1	BrnA antitoxin family protein	-
M1S36_RS19200 (M1S36_19200)	7554..7841	-	288	WP_000438827.1	BrnT family toxin	-
M1S36_RS19205 (M1S36_19205)	8109..8408	-	300	WP_000358348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11194	11194

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T243329 WP_000269904.1 NZ_CP096758:2769-3125 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T243329 NZ_OW970553:1512692-1513174 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT243329 WP_001129974.1 NZ_CP096758:3118-3420 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT243329 NZ_OW970553:1512438-1512704 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1