Detailed information of TA system TA243327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 10528..11186
• Replicon: plasmid p5663
• Accession: NZ_CP096756
• Organism: Acinetobacter baumannii strain 5663

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: M1S57_RS19750
• Protein ID: WP_000312250.1
• Coordinates: 10827..11186 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M1S57_RS19745
• Protein ID: WP_001096429.1
• Coordinates: 10528..10827 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1S57_RS19710 (M1S57_19710)	6586..6843	+	258	WP_071210684.1	hypothetical protein	-
M1S57_RS19715 (M1S57_19715)	6848..7420	+	573	WP_071210685.1	hypothetical protein	-
M1S57_RS19720 (M1S57_19720)	7396..7575	+	180	WP_002081921.1	hypothetical protein	-
M1S57_RS19725 (M1S57_19725)	7592..8221	+	630	WP_071210686.1	hypothetical protein	-
M1S57_RS19730 (M1S57_19730)	8261..8770	+	510	WP_001043200.1	hypothetical protein	-
M1S57_RS19735 (M1S57_19735)	8851..9405	+	555	WP_071210687.1	hypothetical protein	-
M1S57_RS19740 (M1S57_19740)	9455..9991	+	537	WP_000731980.1	hypothetical protein	-
M1S57_RS19745 (M1S57_19745)	10528..10827	-	300	WP_001096429.1	XRE family transcriptional regulator	Antitoxin
M1S57_RS19750 (M1S57_19750)	10827..11186	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M1S57_RS19755 (M1S57_19755)	11387..11953	+	567	WP_000710385.1	hypothetical protein	-
M1S57_RS19760 (M1S57_19760)	12002..12184	+	183	WP_000373385.1	hypothetical protein	-
M1S57_RS19765 (M1S57_19765)	12251..12949	+	699	WP_000873190.1	hypothetical protein	-
M1S57_RS19770 (M1S57_19770)	13088..13471	+	384	WP_071210698.1	hypothetical protein	-
M1S57_RS19775 (M1S57_19775)	13532..14290	+	759	WP_071210697.1	hypothetical protein	-
M1S57_RS19780 (M1S57_19780)	14341..14676	+	336	WP_071210696.1	hypothetical protein	-
M1S57_RS19785 (M1S57_19785)	15543..15857	+	315	WP_071210660.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71044	71044

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T243327 WP_000312250.1 NZ_CP096756:c11186-10827 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T243327 NZ_OW970553:834362-834772 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT243327 WP_001096429.1 NZ_CP096756:c10827-10528 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT243327 NZ_OW970553:834115-834381 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA