Detailed information of TA system TA243295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 25617..26268
• Replicon: plasmid p5745_1
• Accession: NZ_CP096715
• Organism: Acinetobacter baumannii strain 5745

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: M1S38_RS19280
• Protein ID: WP_000269904.1
• Coordinates: 25617..25973 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M1S38_RS19285
• Protein ID: WP_001129974.1
• Coordinates: 25966..26268 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1S38_RS19260 (M1S38_19260)	20861..21803	-	943	Protein_25	replication initiation protein RepM	-
M1S38_RS19265 (M1S38_19265)	22400..23569	-	1170	WP_002134206.1	MobA/MobL family protein	-
M1S38_RS19270 (M1S38_19270)	23874..24635	+	762	WP_000425105.1	hypothetical protein	-
M1S38_RS19275 (M1S38_19275)	24752..25051	+	300	WP_000358348.1	hypothetical protein	-
M1S38_RS19280 (M1S38_19280)	25617..25973	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M1S38_RS19285 (M1S38_19285)	25966..26268	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
M1S38_RS19290 (M1S38_19290)	26261..26470	+	210	WP_000069474.1	hypothetical protein	-
M1S38_RS19295 (M1S38_19295)	26796..27256	-	461	Protein_32	DIP1984 family protein	-
M1S38_RS19300 (M1S38_19300)	27561..29971	-	2411	Protein_33	TonB-dependent receptor ZnuD2	-
M1S38_RS19305 (M1S38_19305)	30071..30412	-	342	WP_200165042.1	BrnA antitoxin family protein	-
M1S38_RS19310 (M1S38_19310)	30399..30686	-	288	WP_000438827.1	BrnT family toxin	-
M1S38_RS19315 (M1S38_19315)	30954..31253	-	300	WP_000358348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..44752	44752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T243295 WP_000269904.1 NZ_CP096715:25617-25973 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T243295 NZ_OW970515:c116606-116301 [Klebsiella pneumoniae]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT243295 WP_001129974.1 NZ_CP096715:25966-26268 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT243295 NZ_OW970515:c116824-116606 [Klebsiella pneumoniae]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Structures

Toxin

• AlphaFold DB: N8YFZ1