Detailed information of TA system TA243291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3226..3877
• Replicon: plasmid p5745_1
• Accession: NZ_CP096715
• Organism: Acinetobacter baumannii strain 5745

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: M1S38_RS19150
• Protein ID: WP_000269904.1
• Coordinates: 3226..3582 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M1S38_RS19155
• Protein ID: WP_001129974.1
• Coordinates: 3575..3877 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1S38_RS19135 (M1S38_19135)	621..1556	+	936	WP_001292329.1	replication initiation protein RepM	-
M1S38_RS19140 (M1S38_19140)	1634..2152	+	519	WP_000839337.1	plasmid replication DNA-binding protein	-
M1S38_RS19145 (M1S38_19145)	2362..2661	+	300	WP_000358348.1	hypothetical protein	-
M1S38_RS19150 (M1S38_19150)	3226..3582	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M1S38_RS19155 (M1S38_19155)	3575..3877	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
M1S38_RS19160 (M1S38_19160)	3870..4079	+	210	WP_000069474.1	hypothetical protein	-
M1S38_RS19165 (M1S38_19165)	4405..4866	-	462	WP_000761346.1	DIP1984 family protein	-
M1S38_RS19170 (M1S38_19170)	5170..7577	-	2408	Protein_7	TonB-dependent receptor ZnuD2	-
M1S38_RS19175 (M1S38_19175)	7705..8019	-	315	WP_001983304.1	BrnA antitoxin family protein	-
M1S38_RS19180 (M1S38_19180)	8006..8293	-	288	WP_000438827.1	BrnT family toxin	-
M1S38_RS19185 (M1S38_19185)	8561..8860	-	300	WP_000358348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..44752	44752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T243291 WP_000269904.1 NZ_CP096715:3226-3582 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T243291 NZ_OW970514:c4638889-4638356 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT243291 WP_001129974.1 NZ_CP096715:3575-3877 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT243291 NZ_OW970514:c4639166-4638900 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1