Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2843038..2843540 Replicon chromosome
Accession NC_013361
Organism Escherichia coli O26:H11 str. 11368

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECO26_RS15315 Protein ID WP_000767829.1
Coordinates 2843038..2843292 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECO26_RS15320 Protein ID WP_001259255.1
Coordinates 2843289..2843540 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO26_RS15290 2838053..2838280 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ECO26_RS15295 2838294..2839352 - 1059 WP_000492324.1 transport protein YeeE -
ECO26_RS15300 2839531..2840889 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECO26_RS34080 2840879..2840941 - 63 WP_010723108.1 membrane protein YoeI -
ECO26_RS15305 2841156..2842085 - 930 WP_000803351.1 LysR family transcriptional regulator -
ECO26_RS15310 2842131..2842955 - 825 WP_000754737.1 SDR family oxidoreductase -
ECO26_RS15315 2843038..2843292 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECO26_RS15320 2843289..2843540 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECO26_RS34085 2843823..2843870 + 48 WP_001375657.1 his operon leader peptide -
ECO26_RS15325 2844016..2844915 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECO26_RS15330 2844921..2846225 + 1305 WP_000009589.1 histidinol dehydrogenase -
ECO26_RS15335 2846222..2847292 + 1071 WP_000108914.1 histidinol-phosphate transaminase -
ECO26_RS15340 2847292..2848359 + 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T24329 WP_000767829.1 NC_013361:c2843292-2843038 [Escherichia coli O26:H11 str. 11368]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T24329 NC_013361:c2843292-2843038 [Escherichia coli O26:H11 str. 11368]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT24329 WP_001259255.1 NC_013361:c2843540-2843289 [Escherichia coli O26:H11 str. 11368]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT24329 NC_013361:c2843540-2843289 [Escherichia coli O26:H11 str. 11368]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References