Detailed information of TA system TA243287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4851..5502
• Replicon: plasmid p5759_3
• Accession: NZ_CP096713
• Organism: Acinetobacter baumannii strain 5759

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: M1S21_RS20630
• Protein ID: WP_000269904.1
• Coordinates: 4851..5207 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M1S21_RS20635
• Protein ID: WP_001129974.1
• Coordinates: 5200..5502 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1S21_RS20610 (M1S21_20610)	101..1036	-	936	WP_001292329.1	replication initiation protein RepM	-
M1S21_RS20615 (M1S21_20615)	1634..2803	-	1170	WP_002134206.1	MobA/MobL family protein	-
M1S21_RS20620 (M1S21_20620)	3108..3869	+	762	WP_000425105.1	hypothetical protein	-
M1S21_RS20625 (M1S21_20625)	3986..4285	+	300	WP_000358348.1	hypothetical protein	-
M1S21_RS20630 (M1S21_20630)	4851..5207	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M1S21_RS20635 (M1S21_20635)	5200..5502	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
M1S21_RS20640 (M1S21_20640)	5495..5704	+	210	WP_000069474.1	hypothetical protein	-
M1S21_RS20645 (M1S21_20645)	6030..6491	-	462	WP_000761346.1	DIP1984 family protein	-
M1S21_RS20650 (M1S21_20650)	6796..9204	-	2409	Protein_9	TonB-dependent receptor ZnuD2	-
M1S21_RS20655 (M1S21_20655)	9332..9644	-	313	Protein_10	BrnA antitoxin family protein	-
M1S21_RS20660 (M1S21_20660)	9631..9917	-	287	Protein_11	BrnT family toxin	-
M1S21_RS20665 (M1S21_20665)	10185..10484	-	300	WP_000358348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33550	33550

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T243287 WP_000269904.1 NZ_CP096713:4851-5207 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T243287 NZ_OW970514:1502598-1503080 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT243287 WP_001129974.1 NZ_CP096713:5200-5502 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT243287 NZ_OW970514:1502344-1502610 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1