Detailed information of TA system TA243274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 14061..14712
• Replicon: plasmid p5773_1
• Accession: NZ_CP096694
• Organism: Acinetobacter baumannii strain 5773

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: M1S18_RS19210
• Protein ID: WP_000269904.1
• Coordinates: 14061..14417 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M1S18_RS19215
• Protein ID: WP_001129974.1
• Coordinates: 14410..14712 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1S18_RS19185 (M1S18_19185)	9318..10251	-	934	Protein_12	replication initiation protein RepM	-
M1S18_RS19190 (M1S18_19190)	10849..11022	-	174	WP_228200477.1	hypothetical protein	-
M1S18_RS19195 (M1S18_19195)	11205..12017	-	813	WP_249377532.1	MobA/MobL family protein	-
M1S18_RS19200 (M1S18_19200)	12321..13080	+	760	Protein_15	hypothetical protein	-
M1S18_RS19205 (M1S18_19205)	13197..13496	+	300	WP_000358348.1	hypothetical protein	-
M1S18_RS19210 (M1S18_19210)	14061..14417	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M1S18_RS19215 (M1S18_19215)	14410..14712	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
M1S18_RS19220 (M1S18_19220)	14705..14914	+	210	WP_000069474.1	hypothetical protein	-
M1S18_RS19225 (M1S18_19225)	15240..15702	-	463	Protein_20	DIP1984 family protein	-
M1S18_RS19230 (M1S18_19230)	16007..18419	-	2413	Protein_21	TonB-dependent receptor ZnuD2	-
M1S18_RS19235 (M1S18_19235)	18547..18861	-	315	WP_001983304.1	BrnA antitoxin family protein	-
M1S18_RS19240 (M1S18_19240)	18848..19135	-	288	WP_000438827.1	BrnT family toxin	-
M1S18_RS19245 (M1S18_19245)	19403..19702	-	300	WP_000358348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..22354	22354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T243274 WP_000269904.1 NZ_CP096694:14061-14417 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T243274 NZ_OW970492:1515022-1515504 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT243274 WP_001129974.1 NZ_CP096694:14410-14712 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT243274 NZ_OW970492:1514768-1515034 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1