Detailed information of TA system TA243262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 14081..14732
• Replicon: plasmid p5955
• Accession: NZ_CP096683
• Organism: Acinetobacter baumannii strain 5955

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: M1S44_RS19750
• Protein ID: WP_000269904.1
• Coordinates: 14081..14437 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M1S44_RS19755
• Protein ID: WP_001129974.1
• Coordinates: 14430..14732 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1S44_RS19730 (M1S44_19730)	9717..10886	+	1170	WP_002134206.1	MobA/MobL family protein	-
M1S44_RS19735 (M1S44_19735)	11481..12412	+	932	Protein_12	replication initiation protein RepM	-
M1S44_RS19740 (M1S44_19740)	12490..13006	+	517	Protein_13	plasmid replication DNA-binding protein	-
M1S44_RS19745 (M1S44_19745)	13216..13515	+	300	WP_000358348.1	hypothetical protein	-
M1S44_RS19750 (M1S44_19750)	14081..14437	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M1S44_RS19755 (M1S44_19755)	14430..14732	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
M1S44_RS19760 (M1S44_19760)	14725..14934	+	210	WP_000069474.1	hypothetical protein	-
M1S44_RS19765 (M1S44_19765)	15260..15721	-	462	WP_000761346.1	DIP1984 family protein	-
M1S44_RS19770 (M1S44_19770)	16026..18434	-	2409	Protein_19	TonB-dependent receptor ZnuD2	-
M1S44_RS19775 (M1S44_19775)	18562..18876	-	315	WP_001983304.1	BrnA antitoxin family protein	-
M1S44_RS19780 (M1S44_19780)	18863..19150	-	288	WP_000438827.1	BrnT family toxin	-
M1S44_RS19785 (M1S44_19785)	19418..19717	-	300	WP_000358348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..22367	22367

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T243262 WP_000269904.1 NZ_CP096683:14081-14437 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T243262 NZ_OW970486:1513347-1513829 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT243262 WP_001129974.1 NZ_CP096683:14430-14732 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT243262 NZ_OW970486:1513093-1513359 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1