Detailed information of TA system TA243256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 21533..22173
• Replicon: plasmid unnamed1
• Accession: NZ_CP096668
• Organism: Yersinia pestis EV76-CN

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: M0M60_RS21355
• Protein ID: WP_002225538.1
• Coordinates: 21533..21874 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q9S1D9
• Locus tag: M0M60_RS21360
• Protein ID: WP_002213446.1
• Coordinates: 21874..22173 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0M60_RS21340 (M0M60_21305)	17233..17427	-	195	WP_002213433.1	Rop family plasmid primer RNA-binding protein	-
M0M60_RS21345 (M0M60_21310)	18464..19246	-	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
M0M60_RS21350 (M0M60_21315)	19243..20265	-	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
M0M60_RS21355 (M0M60_21320)	21533..21874	+	342	WP_002225538.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M0M60_RS21360 (M0M60_21325)	21874..22173	+	300	WP_002213446.1	XRE family transcriptional regulator	Antitoxin
M0M60_RS21365 (M0M60_21330)	22360..23298	-	939	WP_002218234.1	omptin family plasminogen activator Pla	-
M0M60_RS21370 (M0M60_21335)	23541..23847	-	307	Protein_23	hypothetical protein	-
M0M60_RS21375 (M0M60_21340)	24076..25149	+	1074	WP_002218509.1	pesticin	-
M0M60_RS21380 (M0M60_21345)	25184..25610	-	427	Protein_25	pesticin immunity protein	-
M0M60_RS21385 (M0M60_21350)	26846..27040	-	195	WP_002213433.1	Rop family plasmid primer RNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	pla / pla / pla / pla / pla / pla	1..47984	47984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (10-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13288.22 Da
• Isoelectric Point: 8.8337

>T243256 WP_002225538.1 NZ_CP096668:21533-21874 [Yersinia pestis EV76-CN]
MVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAIVL
CAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK

Nucleotide

• Length: 342 bp

>T243256 NZ_OW970479:c115377-115072 [Klebsiella pneumoniae]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11127.94 Da
• Isoelectric Point: 5.8900

>AT243256 WP_002213446.1 NZ_CP096668:21874-22173 [Yersinia pestis EV76-CN]
MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGRRVAFHV

Nucleotide

• Length: 300 bp

>AT243256 NZ_OW970479:c115595-115377 [Klebsiella pneumoniae]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A080J016