Detailed information of TA system TA243255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 11918..12558
• Replicon: plasmid unnamed1
• Accession: NZ_CP096668
• Organism: Yersinia pestis EV76-CN

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: M0M60_RS21310
• Protein ID: WP_002225538.1
• Coordinates: 11918..12259 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q9S1D9
• Locus tag: M0M60_RS21315
• Protein ID: WP_002213446.1
• Coordinates: 12259..12558 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0M60_RS21295 (M0M60_21260)	7621..7813	-	193	Protein_8	Rop family plasmid primer RNA-binding protein	-
M0M60_RS21300 (M0M60_21265)	8850..9632	-	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
M0M60_RS21305 (M0M60_21270)	9629..10651	-	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
M0M60_RS21310 (M0M60_21275)	11918..12259	+	342	WP_002225538.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M0M60_RS21315 (M0M60_21280)	12259..12558	+	300	WP_002213446.1	XRE family transcriptional regulator	Antitoxin
M0M60_RS21320 (M0M60_21285)	12745..13683	-	939	WP_002218234.1	omptin family plasminogen activator Pla	-
M0M60_RS21325 (M0M60_21290)	13926..14231	-	306	WP_002221218.1	hypothetical protein	-
M0M60_RS21330 (M0M60_21295)	14460..15535	+	1076	Protein_15	pesticin	-
M0M60_RS21335 (M0M60_21300)	15570..15967	-	398	Protein_16	pesticin immunity protein	-
M0M60_RS21340 (M0M60_21305)	17233..17427	-	195	WP_002213433.1	Rop family plasmid primer RNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	pla / pla / pla / pla / pla / pla	1..47984	47984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (10-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13288.22 Da
• Isoelectric Point: 8.8337

>T243255 WP_002225538.1 NZ_CP096668:11918-12259 [Yersinia pestis EV76-CN]
MVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAIVL
CAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK

Nucleotide

• Length: 342 bp

>T243255 NZ_OW970478:c5229642-5229259 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11127.94 Da
• Isoelectric Point: 5.8900

>AT243255 WP_002213446.1 NZ_CP096668:12259-12558 [Yersinia pestis EV76-CN]
MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGRRVAFHV

Nucleotide

• Length: 300 bp

>AT243255 NZ_OW970478:c5229884-5229642 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A080J016