Detailed information of TA system TA243233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4894527..4895122
• Replicon: chromosome
• Accession: NZ_CP096664
• Organism: Pseudomonas aeruginosa strain PAO1_Kat-X-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: M0418_RS22790
• Protein ID: WP_003113526.1
• Coordinates: 4894844..4895122 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M0418_RS22785
• Protein ID: WP_003113527.1
• Coordinates: 4894527..4894832 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0418_RS22755 (M0418_22750)	4889660..4890508	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
M0418_RS22765 (M0418_22760)	4890675..4891616	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
M0418_RS22770 (M0418_22765)	4891733..4892347	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
M0418_RS22775 (M0418_22770)	4892389..4892973	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
M0418_RS22780 (M0418_22775)	4893014..4894114	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
M0418_RS22785 (M0418_22780)	4894527..4894832	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
M0418_RS22790 (M0418_22785)	4894844..4895122	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M0418_RS22795 (M0418_22790)	4895175..4895303	-	129	Protein_4503	integrase	-
M0418_RS22800 (M0418_22795)	4895451..4897679	+	2229	WP_003113525.1	TonB-dependent receptor	-
M0418_RS22805 (M0418_22800)	4897749..4898396	-	648	WP_003095021.1	carbonate dehydratase	-
M0418_RS22810 (M0418_22805)	4898458..4899696	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T243233 WP_003113526.1 NZ_CP096664:c4895122-4894844 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T243233 NZ_OW970452:1488637-1489053 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT243233 WP_003113527.1 NZ_CP096664:c4894832-4894527 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT243233 NZ_OW970452:1488410-1488640 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: V6ALY3