Detailed information of TA system TA243206

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1585..2011
• Replicon: plasmid pEC6622-2
• Accession: NZ_CP096589
• Organism: Escherichia coli strain EC6622

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: M1B75_RS24540
• Protein ID: WP_001372321.1
• Coordinates: 1585..1710 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	1787..2011 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1B75_RS24520 (376)	376..663	-	288	WP_000107535.1	hypothetical protein	-
M1B75_RS24525 (688)	688..894	-	207	WP_000547939.1	hypothetical protein	-
M1B75_RS24530 (964)	964..1137	+	174	Protein_3	hypothetical protein	-
M1B75_RS24535 (1135)	1135..1365	-	231	WP_071587244.1	hypothetical protein	-
M1B75_RS24540 (1585)	1585..1710	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
M1B75_RS24545 (1652)	1652..1801	-	150	Protein_6	plasmid maintenance protein Mok	-
- (1787)	1787..2011	-	225	NuclAT_0	-	Antitoxin
- (1787)	1787..2011	-	225	NuclAT_0	-	Antitoxin
- (1787)	1787..2011	-	225	NuclAT_0	-	Antitoxin
- (1787)	1787..2011	-	225	NuclAT_0	-	Antitoxin
M1B75_RS24550 (1823)	1823..2011	+	189	WP_001299721.1	hypothetical protein	-
M1B75_RS24555 (1980)	1980..2742	-	763	Protein_8	plasmid SOS inhibition protein A	-
M1B75_RS24560 (2739)	2739..3173	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
M1B75_RS24565 (3228)	3228..5186	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
M1B75_RS24570 (5245)	5245..5478	-	234	WP_000006004.1	DUF905 domain-containing protein	-
M1B75_RS24575 (5534)	5534..6061	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
M1B75_RS24580 (6534)	6534..6818	-	285	WP_124767219.1	hypothetical protein	-
M1B75_RS24585 (6704)	6704..6970	-	267	WP_001697809.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / tet(A) / blaTEM-1B / sul1 / qacE / ant(3'')-Ia / dfrA1	iroB / iroC / iroD / iroE / iroN	1..140101	140101

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T243206 WP_001372321.1 NZ_CP096589:c1710-1585 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T243206 NZ_OW970436:1497820-1498236 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT243206 NZ_CP096589:c2011-1787 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin