Detailed information of TA system TA243204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4550934..4551536
• Replicon: chromosome
• Accession: NZ_CP096587
• Organism: Escherichia coli strain EC6622

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: M1B75_RS22125
• Protein ID: WP_000897305.1
• Coordinates: 4551225..4551536 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M1B75_RS22120
• Protein ID: WP_000356397.1
• Coordinates: 4550934..4551224 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1B75_RS22095 (4546859)	4546859..4547761	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
M1B75_RS22100 (4547758)	4547758..4548393	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
M1B75_RS22105 (4548390)	4548390..4549319	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
M1B75_RS22110 (4549649)	4549649..4549891	-	243	WP_001087409.1	protein YiiF	-
M1B75_RS22115 (4550111)	4550111..4550329	-	219	WP_001315930.1	CopG family transcriptional regulator	-
M1B75_RS22120 (4550934)	4550934..4551224	-	291	WP_000356397.1	NadS family protein	Antitoxin
M1B75_RS22125 (4551225)	4551225..4551536	-	312	WP_000897305.1	hypothetical protein	Toxin
M1B75_RS22130 (4551765)	4551765..4552673	+	909	WP_001162704.1	alpha/beta hydrolase	-
M1B75_RS22135 (4552737)	4552737..4553678	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
M1B75_RS22140 (4553723)	4553723..4554160	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
M1B75_RS22145 (4554157)	4554157..4555029	-	873	WP_000920762.1	virulence factor BrkB family protein	-
M1B75_RS22150 (4555023)	4555023..4555622	-	600	WP_001295269.1	glucose-1-phosphatase	-
M1B75_RS22155 (4555721)	4555721..4556506	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T243204 WP_000897305.1 NZ_CP096587:c4551536-4551225 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T243204 NZ_OW970436:356817-357164 [Klebsiella pneumoniae]
ATGTGGGATGTGGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATAAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT243204 WP_000356397.1 NZ_CP096587:c4551224-4550934 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT243204 NZ_OW970436:357164-357463 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7